Amg 900

Manufactured by Selleck Chemicals

Sourced in China, United States, United Kingdom

The AMG 900 is a compact and versatile laboratory equipment designed for various research and analytical applications. It features a durable construction and precise temperature control for consistent and reliable performance. The core function of the AMG 900 is to provide a controlled environment for experiments and sample preparation.

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Lab products found in correlation

7 protocols using amg 900

Solubilization of Small Molecules

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Mecamylamine (Sigma Aldrich), Canertinib (AK Scientific, Union City, CA), Bortezomib (AK Scientific), Tretinoin (AK Scientific), and AMG900 (Selleckchem, Houston, TX) were resuspended in dimethyl sulfoxide (DMSO – Canertinib, Bortezomib, Tretinoin, AMG900) or 200 proof ethanol (EtOH – Mecamylamine) at a concentration of 10 mM, stored at -20°C, and used at the indicated concentrations.

Wathieu H., Issa N.T., Fernandez A.I., Mohandoss M., Tiek D.M., Franke J.L., Byers S.W., Riggins R.B, & Dakshanamurthy S. (2017). Differential prioritization of therapies to subtypes of triple negative breast cancer using a systems medicine method. Oncotarget, 8(54), 92926-92942.

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In Vitro Endometrial Decidualization Assay

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The immortalized human endometrial stromal cell line (ATCC, CRL-4003^TM) cells were cultured as previously described [66 (link)]. Human endometrial stromal cells were seeded in 12-well plates and cultured with DMEM/F12 (Merck) containing 10% cFBS (Biological Industries) until ~80% confluency at 37 °C and 5% CO₂. In vitro decidualization was performed as previously described [67 (link)]. Briefly, human endometrial stromal cells were induced by 100 or 500 μM dibutyryl cyclic adenosine monophosphate (db-cAMP, D0627, Merck) and 1 μM medroxyprogesterone acetate (MPA, M1629, Merck) for indicated times. Insulin-like growth factor binding protein-1 (IGFBP1) and prolactin (PRL) are reliable markers for evaluating human in vitro decidualization [67 (link)]. The reagents used in this study included Tubastatin A (TubA, S8049, Selleck Chemicals, Houston, TX, USA), ciliobrevin A (CBA, S8249, Selleck Chemicals), ammonium sulfate ((NH₄)₂SO₄, A610060, Sangon, China), BPV (HOpic) (S8651, Selleck Chemicals), LY294002 (S1105, Selleck Chemicals), AMG-900 (S2719, Selleck Chemicals), TC-S 7010 (S1451, Selleck Chemicals), and prostaglandin E₂ (PGE2, 14010, Cayman chemical).

Li B., Yan Y.P., Liang C., He Y.Y., Wang Y., Li M.Y., Chen S.T., Li Y., Liu A.X., Yan G.J, & Yang Z.M. (2022). Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization. International Journal of Molecular Sciences, 23(24), 15573.

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Evaluating Novel AKI Inhibitors

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AKIs include AMG 900 (in-house synthesis), MLN8054/MLN8237 (Selleck Chemicals), and AZD1152-hQPA (in-house synthesis); molecular structures have previously been reported (25 (link)). Test compounds include Ara-C (Sigma), daunorubicin (Sigma), docetaxel (Sanofi-Aventis), nocodazole (Sigma), nutlin-3a (Sigma), and GF120918 (Sigma) (30 (link)).

Payton M., Cheung H.K., Ninniri M.S., Marinaccio C., Wayne W.C., Hanestad K., Crispino J.D., Juan G, & Coxon A. (2018). Dual targeting of aurora kinases with AMG 900 exhibits potent preclinical activity against acute myeloid leukemia with distinct post-mitotic outcomes. Molecular cancer therapeutics, 17(12), 2575-2585.

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Evaluating Cytotoxic Compounds in Cells

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AMG 900 (catalog number S2719), Alisertib (catalog number S1133) and PNU-74654 (catalog number S8429) were purchased from Selleckchem (Houston, TX). Dilutions were performed following to the manufacturer’s protocol.

Maria A.G., Borges K.S., Lira R.C., Thomé C.H., Berthon A., Drougat L., Kiseljak-Vassiliades K., Wierman M.E., Faucz F.R., Faça V.M., Tone L.G, & Stratakis C.A. (2021). Inhibition of Aurora kinase A activity enhances the antitumor response of beta- catenin blockade in human adrenocortical cancer cells. Molecular and cellular endocrinology, 528, 111243.

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AMG900 and BI2536 Treatment Assay

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Cells were treated with AMG900 (Selleck Chemicals, Houston, TX, USA; S2719) and BI2536 (Selleck Chemicals; S1109) for the indicated times. AMG900 and BI2536 were dissolved in DMSO as a vehicle. The final concentration of vehicle in culture medium was 0.1% (V/V).

Ryu J., Pyo J., Lee C, & Kim J. (2018). An Aurora kinase inhibitor, AMG900, inhibits glioblastoma cell proliferation by disrupting mitotic progression. Cancer Medicine, 7(11), 5589-5603.

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Cell Cycle Regulators and DNA Damage

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We used the following chemical inhibitors: ZM447439 (Selleckchem, Houston, TX) at 2.5 μM, AMG 900 (Selleckchem) at 2 μM, Nutlin-3 (Sigma-Aldrich) at 2.5 μM, Bleomycin (BLEOCIN, EMD Millipore) at 3 μg/ml, and RO-3306 (Tocris, Bristol, UK) at 10 μM.

Potapova T.A., Seidel C.W., Box A.C., Rancati G, & Li R. (2016). Transcriptome analysis of tetraploid cells identifies cyclin D2 as a facilitator of adaptation to genome doubling in the presence of p53. Molecular Biology of the Cell, 27(20), 3065-3084.

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Genotoxicity Evaluation of Pharmaceutical Compounds

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Dimethyl sulfoxide (DMSO, CAS no. 67‐68‐5), methotrexate (MTX, CAS no. 59‐05‐2), mebendazole (MEB, CAS no. 446‐86‐6), carbonyl cyanide m‐chlorophenyl hydrazone (CAS no. 555‐60‐2), carbendazim (CAS no. 2068‐78‐2), and methyl methanesulfonate (MMS, CAS no. 66‐27‐3) were purchased from Millipore Sigma, St. Louis, MO. AMG‐900 (CAS no. 877399‐52‐5) and epothilone A (CAS no. 152044‐53‐6) were from Selleckchem, Houston, TX. NTP BCE (lot number 3012872) was procured from PlusPharma (Vista, CA) and BC XRM (lot number ASB‐00030148‐005) was procured from ChromaDex (Irvine, CA). These samples of NTP BCE and BC XRM are the same materials that were tested by Smith‐Roe et al. (2018).
TK6 cells were purchased from ATCC® (cat. no. CRL‐8015) and grown in a humidified atmosphere at 37°C with 5% CO₂ at or below 1 x 10⁶ cells/mL. The culture medium consisted of RPMI 1640 with 200 μg/mL sodium pyruvate (both from Sigma‐Aldrich, St. Louis, MO), 200 μM L‐glutamine, 50 units/mL penicillin and 50 μg/mL streptomycin (from Mediatech, Manassas, VA), and 10% v/v heat‐inactivated horse serum (Gibco®, a Thermo Fisher Scientific Company, Waltham, MA).

Bernacki D.T., Bryce S.M., Bemis J.C., Dertinger S.D., Witt K.L, & Smith‐Roe S.L. (2019). Evidence for an Aneugenic Mechanism of Action for Micronucleus Induction by Black Cohosh Extract. Environmental and Molecular Mutagenesis, 60(9), 845-856.

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