Dialysis tubing technique was used to purify the synthesized nano-formulations GA for eliminating impurities and free non-conjugated compounds suspended in the solution by eluting it through regenerated cellulose (Amicon 10,000 MWCO ultra filter, Millipore, USA). The Entrapment efficiency (EE%) for GA was measured and processed with the microplate reader (BMG Labtech, Germany). The compound entrapment efficiency was calculated from the ratio of the compound amount incorporated into the NPs to total added compound amount.
Amicon 10 000 mwco ultra filter
Manufactured by Merck Group
Sourced in United States
The Amicon 10,000 MWCO ultra filter is a laboratory filtration device used for the separation and concentration of macromolecules, such as proteins and nucleic acids, based on their molecular weight. It operates by applying pressure or centrifugal force to push a sample solution through a semi-permeable membrane, allowing smaller molecules to pass through while retaining the larger molecules of interest.
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Lab products found in correlation
4 protocols using amicon 10 000 mwco ultra filter
1
Dialysis Purification of Nano-Formulations
2
Quantifying Encapsulated Drug in Nanoparticles
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The EE% of the VRM NPs and APT-VRM NPs was performed to determine the encapsulated VRM in the nanoparticles. The prepared NPs were introduced to a dialysis tube (Amicon 10,000 MWCO ultrafilter, Millipore, USA) for eliminating the impurities and the free non-entrapped VRM.
3
Gallic Acid Calibration Curve and Nanocomposite Characterization
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Calibration curve was generated for gallic acid using serial dilutions of each compound (0, 10, 20, 50, 100 µM). All data were equipped and processed with the microplate reader (BMG Labtech, Germany). The compound entrapment efficiency (EE) was calculated from the ratio of the compound amount incorporated into the nanocomposite to the total added compound amount. The prepared nano-structures were enrolled to dialysis tubing technique for eliminating the impurities and the free non-conjugated compounds suspended in the solution by eluting it through regenerated cellulose (Amicon 10,000 MWCO ultra filter, Millipore, USA).
4
Dialysis Tubing Purification of NPs
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Dialysis tubing technique was used to purify the synthesized NPs for eliminating the impurities and the free non-conjugated compounds suspended in the solution by eluting it through regenerated cellulose (Amicon 10,000 MWCO ultra filter, Millipore, USA). The Entrapment efficiency (EE%) for the NPs were measured and processed with the microplate reader (BMG Labtech, Germany). The compound entrapment efficiency was calculated from the ratio of the compound amount incorporated into the NPs to the total added compound amount [28] .
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