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Allophycocyanin klrg1

Manufactured by BioLegend

Allophycocyanin KLRG1 is a fluorescent protein marker used in flow cytometry to detect the presence of KLRG1 (Killer Cell Lectin-Like Receptor G1) on the surface of cells. KLRG1 is a marker of T cell and NK cell differentiation and senescence.

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2 protocols using allophycocyanin klrg1

1

Cytometry and Neutralization Assay for Mycobacterial Antigens

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For flow cytometry, we used FITC anti-CD3, PE anti-CD27, PE/Cy7 anti-NKp46, FITC anti-CD8, PE anti-CD11b, PE anti-CD56, allophycocyanin KLRG1, (all from BioLegend). For neutralization, we used mAb to IL-4, IL-7, IL-17, IL-21, or isotype-matched control antibody (eBioscience). Recombinant mouse IL-21 was obtained from eBioscience. We used γ-irradiated M. tb H37Rv (γ-M. tb), ESAT6, and Ag85a (all from BEI Resources), and the BCG Tice strain (Organon USA Inc.).
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2

Cytometry and Neutralization Assay for Mycobacterial Antigens

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry, we used FITC anti-CD3, PE anti-CD27, PE/Cy7 anti-NKp46, FITC anti-CD8, PE anti-CD11b, PE anti-CD56, allophycocyanin KLRG1, (all from BioLegend). For neutralization, we used mAb to IL-4, IL-7, IL-17, IL-21, or isotype-matched control antibody (eBioscience). Recombinant mouse IL-21 was obtained from eBioscience. We used γ-irradiated M. tb H37Rv (γ-M. tb), ESAT6, and Ag85a (all from BEI Resources), and the BCG Tice strain (Organon USA Inc.).
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