Human fabp2 1 fabp duoset elisa

Manufactured by R&D Systems

Sourced in United States

The Human FABP2/I-FABP DuoSet ELISA from R&D Systems is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human Fatty Acid Binding Protein 2 (FABP2) levels in cell culture supernates, serum, and plasma.

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Close spelling variants of this product

DuoSet ELISA (635 citations) ELISAs (144 citations) DuoSets (49 citations) FABP2, (4 citations) Human ELISA DuoSet (2 citations)

4 protocols using human fabp2 1 fabp duoset elisa

Biomarkers of Immune Activation in HIV

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We centrally measured plasma levels of ten soluble biomarkers of all participants. sTNFRII, sCD14, sCD163, CXCL10, I-FABP, and hyaluronic acid were measured by specific ELISA (Human TNF RII/TNFRSF1B DuoSet ELISA, Human CD14 DuoSet ELISA, Human CD163 DuoSet ELISA, Human CXCL10/IP10 DuoSet ELISA, Human FABP2/I-FABP DuoSet ELISA, and Hyaluronan DuoSet ELISA, R&D Systems). IL-17, IL-6, and TNF-α were measured by single-molecule array (SiMoA) assay (Quanterix), and CRP by immunochemistry (CRP LX HS, Cobas C, integra, Roche Diagnostics). Samples with undetectable levels were attributed half the threshold value.
We measured plasma HIV RNA levels of PRIMO participants, using an ultrasensitive real-time PCR technique (GENERIC HIV, Biocentric, France) and total HIV DNA levels from whole blood samples using a real-time PCR assay (GENERIC Biocentric, France) [19] (link).
The data that support the findings of this study are available on request to the corresponding author. The data are not publicly available due to privacy restrictions.

Novelli S., Lécuroux C., Goujard C., Reynes J., Villemant A., Blum L., Essat A., Avettand-Fenoël V., Launay O., Molina J.M., Bourgeois C, & Meyer L. (2020). Persistence of monocyte activation under treatment in people followed since acute HIV-1 infection relative to participants at high or low risk of HIV infection. EBioMedicine, 62, 103129.

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Plasma Biomarker Quantification by ELISA and Multiplex

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The concentration of some of the plasma markers was measured by ELISA using Human sCD14 DuoSet ELISA (R&D Systems, DY383), Human FABP2/I-FABP DuoSet ELISA (R&D Systems, DY3078), and Human Zonulin/Haptoglobin ELISA kit (ARP American Research Products, E01Z0004) per manufacturer’s instructions. Also, Milliplex multiplex assays using Luminex were used to determine the concentration of soluble ST2 and CD163 using the Human Cardiovascular Disease Magnetic bead panel 5 (Milliplex, HCVD5MAG-67K), per manufacturer’s instructions. The concentration of IL-6 and TNF-α was determined using the Human TH17 magnetic Bead Panel (Milliplex, HTH17MAG-14K) per manufacturer’s instructions.

Asowata O.E., Singh A., Ngoepe A., Herbert N., Fardoos R., Reddy K., Zungu Y., Nene F., Mthabela N., Ramjit D., Karim F., Govender K., Ndung’u T., Porterfield J.Z., Adamson J.H., Madela F.G., Manzini V.T., Anderson F., Leslie A, & Kløverpris H.N. (2021). Irreversible depletion of intestinal CD4+ T cells is associated with T cell activation during chronic HIV infection. JCI Insight, 6(22), e146162.

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Plasma Protein Quantification by ELISA

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Frozen plasma aliquots were thawed for ELISA at RT, used once only, with no repeated freeze-thaws allowed, and evaluated immediately. Plasma protein concentration was determined using Human FABP2/I-FABP DuoSet ELISA (for FABP2), Human Trappin-2/Elafin DuoSet ELISA (for PI3), Human Reg3A DuoSet ELISA Reg3a (for REG3A, all from R&D, Minneapolis, MN, USA), Human Occludin (for OCLN), and KRT15/CK15/Cytokeratin 15 ELISA Kit (for KRT15, both from LSBio, Seattle, WA, USA), and a Human Cytokeratin 20 ELISA Kit (for KRT20, obtained from Novus, Littleton, CO, USA). All ELISA tests were executed according to manufacturer’s instructions. Optimal plasma dilutions were pre-determined in pilot experiments. Plasma samples were diluted for distinct ELISA assays, as follows: Reg3α, both GI and cutaneous aGVHD manifestation group 1:1000, other samples 1:30, PI3 1:100, FABP2 1:1, KRT15 1:300, OCLN 1:10 and KRT20 1:1.

Lupsa N., Szegedi Á., Gézsi A., Vuncs Z., Masszi T., Mikala G., Reményi P., Deola S., Lakshmanan A.P., Terranegra A., Buzás E.I, & Pós Z. (2022). Decreased Plasma Level of Cytokeratin 20 (KRT20) Is Indicative of the Emergence and Severity of Acute GvHD Irrespective to the Type of Organ Involvement. Biomedicines, 10(3), 519.

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Schistosoma mansoni Infection Evaluation

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S. mansoni infection was determined by microscopy using the Kato-Katz fecal thick smear method [39 ]. At screening, baseline, 4-week, and 12-month visits, participants provided stool samples collected on two separate days. Slides were prepared and assessed in duplicate for each stool sample within 24 hours of collection. For each visit, the slide concentrations of eggs per gram of stool (EPG) were averaged, and any value equal to or greater than 1 EPG was considered positive. For quality control, 10% of the slides were randomly selected and examined by a senior microscopist at the Instituto René Rachou, FIOCRUZ, in Belo Horizonte, MG. Plasma LPS was quantified using the Pierce LAL Chromogenic Endotoxin Quantitation Kit (Thermo Fisher Scientific, Waltham, MA, USA). Plasma I-FABP and IGF-1 concentrations were measured by enzyme-linked immunosorbent assay (ELISA) using kits from R&D Systems (Human FABP2/I-FABP DuoSet ELISA #DY3078, Human IGF-I/IGF-1 Quantikine ELISA #DG100B). Biomarker measurements were conducted at the Instituto René Rachou, FIOCRUZ in Brazil.

Araújo Fiuza J., Colt S., Gambogi de Ornellas L., Ferreira Matoso L., Gazzinelli A., Friedman J.F, & Corrêa-Oliveira R. (2022). The role of environmental enteric dysfunction in the pathogenesis of Schistosoma mansoni-associated morbidity in school-aged children. PLoS Neglected Tropical Diseases, 16(10), e0010837.

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