Human pancreatic cancer cell lines (PANC-1, Capan-1, Capan-2 and SW1990) and non-tumorigenic human gastric epithelial cells were obtained from the ATCC (Manassas, VA, USA). The cell lines were authenticated by short-tandem repeat analysis. Cell lines were initially expanded and cryopreserved within 1 month of receipt and were typically used for 3 months; and at which time, a fresh vial of cryopreserved cells was used (2013-2015). These cells were maintained in culture as an adherent monolayer in DMEM or IMDM supplemented with 10% FBS.
Brusatol (CAS: 14907-98-3) was isolated from Bruceae Fructus in our laboratory, and its structural identity was confirmed by comparing its NMR and HRMS data with those published previously [12 (link)]. Its purity was determined to exceed 98% by HPLC analysis. All cell culture reagents were purchased from Invitrogen (Grand Island, NY, USA). Gemcitabine (GEM) (Gemzar, Eli Lilly, USA) and 5-Fluorouracil injection (5-FU) (China Food and Drug Administration, China; approval number H12020959) were procured through the Pharmacy of the Clinical Center, Shenzhen People’s Hospital, Guangdong Province, China.
Brusatol (CAS: 14907-98-3) was isolated from Bruceae Fructus in our laboratory, and its structural identity was confirmed by comparing its NMR and HRMS data with those published previously [12 (link)]. Its purity was determined to exceed 98% by HPLC analysis. All cell culture reagents were purchased from Invitrogen (Grand Island, NY, USA). Gemcitabine (GEM) (Gemzar, Eli Lilly, USA) and 5-Fluorouracil injection (5-FU) (China Food and Drug Administration, China; approval number H12020959) were procured through the Pharmacy of the Clinical Center, Shenzhen People’s Hospital, Guangdong Province, China.