Glucose 201 rt system

Manufactured by HemoCue

Sourced in Sweden

The HemoCue® Glucose 201 RT System is a point-of-care device designed to measure glucose levels in whole blood samples. It provides fast and accurate results, enabling healthcare professionals to make timely clinical decisions.

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Lab products found in correlation

Architect i2000sr, by Abbott (1 mentions) K6219, by Agilent Technologies (1 mentions)

Spelling variants of this product

HemoCue Glucose 201+ (24 citations) HemoCue Glucose 201 System (12 citations) HemoCue Glucose 201 RT (11 citations) HemoCue 201+ system (9 citations)

6 protocols using glucose 201 rt system

Postprandial Mesenteric Blood Flow Analysis

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Experimental procedures are outlined in Fig. 1. Participants were instructed to fast for at least seven hours prior to MRI scanning. Phase-contrast magnetic resonance imaging (PC-MRI) was performed on a 1.5T MRI scanner to assess postprandial increases in mesenteric blood flow. Blood glucose concentration was measured from a drop of blood obtained by puncturing a fingertip, while subjects were lying in the MRI scanner, using a glucose meter (HemoCue^® Glucose 201 RT System, Denmark).
Before PC-MRI, participants completed two questionnaires to access subjective non-motor symptoms and gastrointestinal symptoms. Participants were positioned in the MRI scanner and the SMA was located with a scout scan. Two pre-meal baseline measurements of mesenteric blood flow were performed followed by measurement of baseline blood glucose level. Participants were given two minutes to ingest a standardized liquid test meal. They used a straw for meal intake while lying on the bed outside the scanner bore. Immediately after meal intake, we performed blocks of six consecutive blood flow measurements in the SMA using PC-MRI. Each block of blood flow measurements was followed by a new blood glucose measurement. Alternating blood flow and blood glucose measurement were repeated at least four times (Fig. 1).

Siebner T.H., Fugl Madelung C., Bendtsen F., Løkkegaard A., Hove J.D, & Siebner H.R. (2021). Postprandial Increase in Mesenteric Blood Flow is Attenuated in Parkinson’s Disease: A Dynamic PC-MRI Study. Journal of Parkinson's Disease, 11(2), 545-557.

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Blood Sampling and Plasma Insulin Measurement

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To facilitate the sampling of blood and arterialize the composition of the blood samples, one of the arms and hands of the participants were kept out of water by being placed on a foam buoy, as mentioned above, and the fingertip that was targeted for blood sampling was heated using a heated wheat bag (Feel Good Australia, Victoria). Capillary blood glucose from a fingertip was sampled and analyzed using a glucose analyzer (HemoCue® Glucose 201 RT System), as outlined by Dugan and colleagues [26 (link)]. To measure the level of plasma insulin, the arm that had been cannulated was heated for 5 minutes as described above (Feel Good Australia, Victoria) prior to the collection of venous blood via the antecubital vein. The process of preparing venous blood for the analysis of bioactive free insulin is described in Dugan and colleagues [26 (link)]. Plasma insulin levels were assayed by a local clinical laboratory (PathWest, Osborne Park, West Australia, Australia) using the Architect chemiluminescent microparticle immunoassay system (Abbott Architect i2000SR, Illinois, USA).

Abramoff K.J., De Souza L.L., Maloney S.K., Davis E.A., Jones T.W, & Fournier P.A. (2023). Effect of Neck-Deep Immersion in Cool or Thermoneutral Water on Blood Glucose Levels in Individuals With Type 1 Diabetes. Journal of the Endocrine Society, 7(12), bvad128.

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Oral Glucose Tolerance Test Protocol

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A standard 75 g OGTT was performed using 82.5 g glucose monohydrate (Fagron, Netherlands) diluted in 250 ml drinking water and taken by the participant within 5 min. The participant did not take food or other drinks during the two test hours. Glucose was measured fasting, 30 and 120 min after administration of the glucose solution. All Glucose measurements were determined from whole blood and converted to plasma equivalents using the Hemocue Glucose 201 RT system (Hemocue®, Ängelholm, Sweden). Diabetes was defined according to WHO guidelines [18 (link)]. Fasting insulin (μIU/mL) was measured using a commercial ELISA kit (DAKO code K6219, DAKO, Glostrup, Denmark). HOMA-B and HOMA-IR were calculated by using the FPG and fasting insulin using standard formulae [19 (link)]. HbA1c (mmol /mol) was measured in fresh (within 30 min) EDTA stabilized blood on a Quo-test A1C analyzer (Quotient Diagnostics, Surrey, UK). Based on HbA1c, pre-diabetes and diabetes were defined as HbA1c 39 to 47 mol/mol and ≥ 48 mmol/mol, respectively [20 ]. C-reactive protein (CRP) was measured in serum using a latex enhanced immunoturbidimetric assay (HORIBA ABX A11A01611) for Pentra 400 (HORIBA ABC, Montpellier, France).

Amare H., Olsen M.F., Friis H., Kæstel P., Andersen Å.B., Abdissa A., Yilma D., Girma T, & Faurholt-Jepsen D. (2021). Effects of nutritional supplementation on glucose metabolism and insulin function among people with HIV initiating ART. BMC Nutrition, 7, 60.

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Blood lipid and glucose analysis

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A blood sample was drawn from the antecubital vein after overnight fasting. Concentrations of total cholesterol (mmol/L), low-density lipoprotein (LDL) (mmol/L), high-density lipoprotein (HDL) (mmol/L), and triglycerides (mmol/L) were estimated by standard methods at Aleris MediLab (Stockholm, Sweden, ISO/IEC 151 89, certified). Fasting plasma glucose concentrations were analysed at the primary care unit from venous blood sample, using the HemoCue® Glucose 201 RT system.

Lönnberg L., Ekblom-Bak E, & Damberg M. (2020). Reduced 10-year risk of developing cardiovascular disease after participating in a lifestyle programme in primary care. Upsala Journal of Medical Sciences, 125(3), 250-256.

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Measurement of Biomarkers in Clinical Study

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Blood glucose is measured by a validated, portable system at room temperature (HemoCue Glucose 201 RT system (HemoCue AB, Ängelholm, Sweden) at all centres. All other blood or serum measurements will be done at Haukeland University Hospital, Bergen (certified laboratory NSEN-ISO 15189), in frozen samples stored at −80°C. HbA1c is measured in EDTA whole blood samples which have been stored at maximum for 8 weeks by high-performance liquid chromatography (HPLC), (BioRad, Hercules, California, USA). Liver enzymes and plasma lipids are measured using standard methods on a Cobas c702 autoanalyser. Liver enzymes are measured photometrically according to the International Federation of Clinical Chemistry (IFCC) method (Roche Diagnostics, Mannheim, Germany). Serum triacylglycerides and total cholesterol are measured with an enzymatic colorimetric method. Low-density lipoprotein cholesterol (LDL-C) is measured photometrically, and high-density lipoprotein cholesterol (HDL-C) is measured by a homogeneous enzymatic colorimetric method.

Hjorth T., Schadow A., Revheim I., Spielau U., Thomassen L.M., Meyer K., Piotrowski K., Rosendahl-Riise H., Rieder A., Varela P., Lysne V., Ballance S., Koerner A., Landberg R., Buyken A, & Dierkes J. (2022). Sixteen-week multicentre randomised controlled trial to study the effect of the consumption of an oat beta-glucan-enriched bread versus a whole-grain wheat bread on glycaemic control among persons with pre-diabetes: a study protocol of the CarbHealth study. BMJ Open, 12(8), e062066.

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Metabolic Biomarkers in Fasting Venous Blood

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After a minimum of 3 hours of fasting, 2 ml of venous blood was drawn from the antecubital fossa. We determined glucose concentrations from whole blood using the HemoCue Glucose 201 RT System (HemoCue, Ängelholm, Sweden). Glycosylated haemoglobin (HbA1c, mmol/mol) was determined from whole blood using a DCCT aligned Quo-Test A1c Analyzer (EKF Diagnostics, Cardiff, Wales). After clotting, the whole blood was centrifuged to isolate serum, divided into three 0.4-ml aliquots and frozen at −80 °C until analysed at the Ethiopian Public Health Institute, Addis Ababa, Ethiopia. Serum concentrations of total cholesterol, low-density lipoprotein (LDL)–cholesterol, high-density lipoprotein (HDL)–cholesterol, and triglycerides (all in mmol/l) were determined using the COBAS 6000, module c501, and insulin (μU/ml) and C-peptide (ng/ml) concentrations were determined using the COBAS 6000, module e601 (Roche Diagnostics International, Rotkreuz, Switzerland). We calculated the homeostasis model assessment of insulin resistance index (HOMA-IR) as insulin × glucose/22.5 [24 (link)].

Wibaek R., Vistisen D., Girma T., Admassu B., Abera M., Abdissa A., Jørgensen M.E., Kæstel P., Michaelsen K.F., Friis H., Wells J.C, & Andersen G.S. (2019). Associations of fat mass and fat-free mass accretion in infancy with body composition and cardiometabolic risk markers at 5 years: The Ethiopian iABC birth cohort study. PLoS Medicine, 16(8), e1002888.

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