Total cholesterol and total glyceride kits

Chitosan oligosaccharides classified by molecular weight (GO2KA1; MW < 1000 Da) were purchased from Kunpoong Bio Co. Ltd. (Jeju, Korea). Corn starch, casein, vitamin mix, mineral mix, calcium phosphate and sodium chloride were purchased from Raon Bio (Yonginsi, Korea). Total cholesterol and total glyceride kits were purchased from Stanbio laboratory (Boerne, TX, USA). Unless noted, otherwise, all chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The fast SYBR real-time PCR master mix, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), bovine calf newborn serum (BCS), penicillin-streptomycin (P/S), and trypsin-EDTA were obtained from Life Technologies (Grand Island, NY, USA). Adiponectin ELISA kit was purchased from Thermo Fisher Scientific (Invitrogen, Carlsbad, CA, USA). 3T3-L1 cells (ATCCV^®CL-173^TM) were used below passage 12. 3T3-L1 preadipocytes were propagated and cultured in DMEM medium supplemented with 10% BCS and 1% P/S until confluent and maintained for additional 2 days and differentiated as reported previously [26 (link)] with or without GO2KA1.

Chitosan oligosaccharides classified by molecular weight (GO2KA1; MW < 1000 Da) were purchased from Kunpoong Bio Co. Ltd. (Seoul, Korea). Corn starch, casein, vitamin mix, mineral mix, calcium phosphate and sodium chloride were purchased from Raon Bio (Yonginsi, Korea). Total cholesterol and total glyceride kits were purchased from Stanbio laboratory (Boerne, USA). Blood glucose tester was purchased from Caresens (I-SENS. Inc., Anyang, Korea) and HbA1c analyzer was purchase from Infopia Inc. (Anyang, Korea).Unless noted, all chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).

Arginyl-fructose (AF)-enriched barley powder (BEE) was purchased from Kunpoong Bio Co., Ltd. (Jeju, Korea). Corn starch, casein, vitamin mix, mineral mix, calcium phosphate, and sodium chloride were purchased from Raon Bio (Yonginsi, Korea). Total cholesterol and total glyceride kits were purchased from Stanbio laboratory (Boerne, TX, USA). Unless noted, all chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The fast SYBR real-time PCR master mix, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), bovine calf newborn serum (BCS), penicillin-streptomycin (p/S), and trypsin-EDTA were obtained from Life Technologies (Grand Island, NY, USA). Adiponectin ELISA kit was purchased from Thermo Fisher Scientific (Invitrogen, Carlsbad, CA, USA). 3T3-L1 cells (ATCCV^®CL-173TM) were used below passage 12. 3T3-L1 preadipocytes were propagated and cultured in DMEM medium supplemented with 10% BCS and 1% P/S until confluent and maintained for additional 2 days and differentiated as reported previously [26 (link)] with or without BEE.