Tlr4ko

Male C57BL/6 (6-8 weeks old) mice were purchased from Charles River Breeding Laboratories (Wilmington, MA), and allowed to acclimate to the vivarium for 7-10 days prior to experiments. The IL-1R1-deficient IL-1R1^r/r mice were generated by our lab previously (Liu et al., 2015 (link)). Tie2Cre-IL-1R1^r/r and CX3CR1Cre-IL-1R1^r/r mice were generated by crossbreeding IL-1R1^r/r mice with Tie2Cre mice (stock #004128; Jackson Laboratories, Bar Harbor, ME) and CX3CR1Cre mice (STOCK Tg(Cx3cr1-cre)MW126Gsat/Mmucd; MMRRC). Previously, a mouse line containing a floxed IL-1R1 gene (IL-1R1^f/f) was created (Robson et al., 2016 (link)). In the present study, CX3CR1Cre-IL-1R1^f/f mice were generated by crossbreeding IL-1R1^f/f mice (obtained from Randy Blakely, Florida Atlantic University, Jupiter, FL, USA) with CX3CR1Cre mice. TLR2KO (stock #004650; Jackson Laboratories) and TLR4KO (stock #007227; Jackson Laboratories) mice were purchased from Jackson Laboratories. All mice were group housed in 11.5”× 7.5”× 6” polypropylene cages. The animal room was maintained at 21 °C under a 12 h light/dark cycle with rodent chow and water available ad libitum. All procedures were in accordance with the National Institute of Health Guidelines for the Care and Use of Laboratory Animals and were approved by the Ohio State University Institutional Laboratory Animal Care and Use Committee.

C57Bl/6 WT, MyD88KO, Caspase-1KO, IL-1RKO, TLR2KO, TLR7KO and TLR4KO mice were purchased from Jackson Laboratories. TLR9KO mice were kindly provided by Dr. Jillian Poole (University of Nebraska Medical Center) with permission from Shizuo Akira. Mice were housed and bred at the University of Colorado Biological Resource Center and were used at 6-12 weeks of age. All experiments were approved by the Institutional Animal Care and Use Committee of the University of Colorado Denver, in accordance with the NIH guidelines.

All animal studies were carried out in accordance with protocols approved by the Institutional Animal Care and Use Committee in Icahn School of Medicine at Mount Sinai. Six to eight-week-old C57BL/6J (Jackson Laboratory stock #000664), BALB/cJ (Jackson Laboratory Stock #000651), MyD88^KO (Jackson Laboratory stock #009088), TLR2^KO (Jackson Laboratory stock #004650), TLR4^KO (Jackson Laboratory stock #029015) mice were purchased from Jackson Labs. Mice were kept under specific pathogen-free conditions at the Icahn School of Medicine at Mount Sinai Gnotobiotic Facility. Germ free C57BL/6J were bred in isolators at the Icahn School of Medicine at Mount Sinai Gnotobiotic Facility. All mice were provided unrestricted access to sterile water and chow (5K67, LabDiet) and under a strict 12-hour light cycle. Both male and female mice were used and mice were used at 6–8 weeks of age.