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Total jnk

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Total JNK is a laboratory equipment product that measures the total amount of the c-Jun N-terminal kinase (JNK) protein in a sample. It provides a quantitative assessment of the JNK protein levels without making any interpretations or extrapolations about its intended use.

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Lab products found in correlation

Cyp2e1, by Abcam (4 mentions) Hrp conjugated goat anti mouse, by Jackson ImmunoResearch (4 mentions) β actin, by Merck Group (4 mentions) Hrp conjugated goat anti rabbit, by Jackson ImmunoResearch (2 mentions) Total s6, by Cell Signaling Technology (2 mentions) Phospho jnk, by Cell Signaling Technology (2 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (2 mentions) Phospho s6, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Horseradish peroxidase hrp conjugated goat anti rabbit, by Jackson ImmunoResearch (2 mentions) Total 4e bp1, by Cell Signaling Technology (2 mentions) P jnk, by Cell Signaling Technology (2 mentions) P 4ebp1, by Cell Signaling Technology (2 mentions) Phosphorylated p38, by BD (1 mentions) Cnpase, by Merck Group (1 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) Immobilon p filter, by Merck Group (1 mentions) Total erk, by Merck Group (1 mentions) Phosphorylated erk, by BD (1 mentions) Glial fibrillary acidic protein (gfap), by Santa Cruz Biotechnology (1 mentions)

5 protocols using total jnk

1

Antibody Identification in Protein Analysis

Cited 2 times
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The rabbit anti-APAP-AD antibody was a kind gift from Dr. Lance Pohl (National Heart, Lung and Blood Institute, USA).13 (link) The other antibodies were: CYP2E1 (#ab28146) from Abcam (Cambridge, UK), p62 (#H00008878-M01) from Abnova (Taipei, China), phospho-S6 (#4858), total S6 (#2217), phospho-JNK (#4668), and GAPDH (#2118) from Cell Signaling Technology (Danvers, MA, USA); β-actin (#a5441) from Sigma-Aldrich (St. Louis, MO, USA), total JNK (#554285) from BD Pharmingen (Franklin Lakes, NJ, USA); proliferating cell nuclear antigen (PCNA) (#SC-56) from Santa Cruz Biotechnology (Dallas, TX, USA). The rabbit anti-microtubule-associated protein 1 light chain 3 (LC3) antibody was described previously.22 (link) The secondary antibodies were HRP-conjugated goat-anti-rabbit (#111-035-045), and HRP-conjugated goat-anti-mouse (#115-035-062) were from Jackson ImmunoResearch (West Glove, PA, USA). All other reagents were either from Sigma (USA) or Thermo Fisher Scientific (USA).
Sun H., Ni H.M., McCracken J.M., Akakpo J.Y., Fulte S., McKeen T., Jaeschke H., Wang H, & Ding W.X. (2021). Liver-specific deletion of mechanistic target of rapamycin does not protect against acetaminophen-induced liver injury in mice. Liver research, 5(2), 79-87.
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2

Antibody Characterization for APAP Toxicity

Cited 1 time
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The rabbit anti-APAP-AD antibody was a kind gift from Dr. Lance Pohl (National Heart, Lung and Blood Institute).31 (link) The other antibodies were: CYP2E1 (Abcam, #ab28146),p62 (Abnova, #H00008878-M01), phospho-eukaryotic initiation factor 4E-binding protein 1 (p-4EBP1) (Cell Signaling, #9451), total 4EBP1 (Cell Signaling, #9452), p-JNK (Cell Signaling, #4668), total JNK (BD Pharmingen, #554285), β-actin (Sigma, #a5541), and glyceralde-hyde phosphate dehydrogenase (GAPDH) (Cell Signaling, #2118). The microtubule-associated protein 1 light chain 3 (LC3) antibody was developed as described previously.36 The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat-anti-rabbit (Jackson ImmunoResearch, #111–035-045), and HRP-conjugated goat-anti-mouse (Jackson ImmunoResearch, #115-035-062).
Li Y., Ni H.M., Jaeschke H, & Ding W.X. (2019). Chlorpromazine protects against acetaminophen-induced liver injury in mice by modulating autophagy and c-Jun N-terminal kinase activation. Liver research, 3(1), 65-74.
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3

Immunoblotting Analysis of Neural Proteins

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Immunoblotting was carried out as previously reported.4 (link) Brains, optic nerves and cultured oligodendrocytes were freshly isolated, and then homogenized. Samples were separated on a sodium dodecyl sulfate-PAGE and subsequently transferred to an Immobilon-P filter (Millipore, Billerica, MA, USA). Membranes were incubated with an antibody against Dock3 (1 : 1000), Neu-N (1 : 1000, Millipore), NG2 (1 : 500, Millipore), GFAP (1 : 500, Santa Cruz), CNPase (1 : 1000, Sigma), Elmo, total Erk, phosphorylated Erk, total p38, phosphorylated p38, total JNK, phosphorylated JNK or actin (1 : 1000; BD Biosciences).
Namekata K., Kimura A., Harada C., Yoshida H., Matsumoto Y, & Harada T. (2014). Dock3 protects myelin in the cuprizone model for demyelination. Cell Death & Disease, 5(8), e1395-.
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4

Antibody Identification in Protein Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The rabbit anti-APAP-AD antibody was a kind gift from Dr. Lance Pohl (National Heart, Lung and Blood Institute, USA).13 (link) The other antibodies were: CYP2E1 (#ab28146) from Abcam (Cambridge, UK), p62 (#H00008878-M01) from Abnova (Taipei, China), phospho-S6 (#4858), total S6 (#2217), phospho-JNK (#4668), and GAPDH (#2118) from Cell Signaling Technology (Danvers, MA, USA); β-actin (#a5441) from Sigma-Aldrich (St. Louis, MO, USA), total JNK (#554285) from BD Pharmingen (Franklin Lakes, NJ, USA); proliferating cell nuclear antigen (PCNA) (#SC-56) from Santa Cruz Biotechnology (Dallas, TX, USA). The rabbit anti-microtubule-associated protein 1 light chain 3 (LC3) antibody was described previously.22 (link) The secondary antibodies were HRP-conjugated goat-anti-rabbit (#111-035-045), and HRP-conjugated goat-anti-mouse (#115-035-062) were from Jackson ImmunoResearch (West Glove, PA, USA). All other reagents were either from Sigma (USA) or Thermo Fisher Scientific (USA).
Sun H., Ni H.M., McCracken J.M., Akakpo J.Y., Fulte S., McKeen T., Jaeschke H., Wang H, & Ding W.X. (2021). Liver-specific deletion of mechanistic target of rapamycin does not protect against acetaminophen-induced liver injury in mice. Liver research, 5(2), 79-87.
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5

Antibody Characterization for APAP Toxicity

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The rabbit anti-APAP-AD antibody was a kind gift from Dr. Lance Pohl (National Heart, Lung and Blood Institute).31 (link) The other antibodies were: CYP2E1 (Abcam, #ab28146),p62 (Abnova, #H00008878-M01), phospho-eukaryotic initiation factor 4E-binding protein 1 (p-4EBP1) (Cell Signaling, #9451), total 4EBP1 (Cell Signaling, #9452), p-JNK (Cell Signaling, #4668), total JNK (BD Pharmingen, #554285), β-actin (Sigma, #a5541), and glyceralde-hyde phosphate dehydrogenase (GAPDH) (Cell Signaling, #2118). The microtubule-associated protein 1 light chain 3 (LC3) antibody was developed as described previously.36 The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat-anti-rabbit (Jackson ImmunoResearch, #111–035-045), and HRP-conjugated goat-anti-mouse (Jackson ImmunoResearch, #115-035-062).
Li Y., Ni H.M., Jaeschke H, & Ding W.X. (2019). Chlorpromazine protects against acetaminophen-induced liver injury in mice by modulating autophagy and c-Jun N-terminal kinase activation. Liver research, 3(1), 65-74.
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