Sodium hypochlorite solution naocl

Dulbecco's Modified Eagle's Medium (DMEM; GIBCO), antibiotics like penicillin (GIBCO), and streptomycin (GIBCO) were purchased from Invitrogen, U.K. Fetal Calf Serum (FCS; GIBCO), glutamine^® (GIBCO), and sodium pyruvate (GIBCO) were purchased from Invitrogen USA. Acetic acid, zinc (II) acetate (Zn(CH₃COO)₂ * 2H₂O ≤ 100%), copper (II) chloride (CuCl₂ * 2H₂O ≥ 99%) and sodium hydroxide (NaOH ≥ 99.0%) were purchased from Merck. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), sodium hypochlorite solution (NaOCL, available chlorine 10–15%), and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich.

The following reagents were employed: acetone was acquired from Labsynth (Diadema, SP, Brazil), hexane, ethanol, methanol and methyl tert-butyl ether (MTBE) were acquired from Merck (Darmstadt, Germany), vanillin and sulfuric acid (H₂SO₄) were obtained from Dinâmica Química Contemporânea (Diadema, SP, Brazil), and the Folin–Ciocalteu reagent, sodium carbonate Na₂CO₃, dithiothreitol (DTT), metaphosphoric acid, potassium chloride (KCl), Tris (hydroxymethyl) aminomethane, potassium peroxydisulfate, 2,20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), monobasic and dibasic potassium phosphate, fluorescein sodium salt, 2,20-azobis(2-methylpropionamidine) dihydrochloride (AAPH), sodium hypochlorite solution (NaOCl) and rhodamine 123 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid, ascorbic acid, lutein, zeaxanthin, β-cryptoxanthin, α-carotene β-carotene, catechin, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-car- boxylic acid (Trolox) were used as standards and purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was obtained from a Millipore Milli-Q System (Merck Millipore, Steinfurt, Germany).