Morphometric measurements were completed using cross-sectional measurements of Masson’s Trichrome-stained tissue samples (Axiovison v. 3.1, Zeiss). Rabbits received bromodeoxyuridine (BrdU) injections (50mg/kg) 24 hours prior to graft harvest. Proliferating cells were identified with a BrdU antibody (Invitrogen) according to the manufacturer’s protocol, and visualized using DAB staining. Density of actively proliferating cells (BrdU-positive cells per unit area) was determined for the neointima and media. SPSS 22 software (SPSS Inc, Chicago, Il) was used for statistical analysis. Comparisons were done using ANOVA and t-test where appropriate, with P < .05 considered significant.
Masson s trichrome
Manufactured by Zeiss
Sourced in Germany
Masson's Trichrome is a histological staining technique used to differentiate various types of connective tissue, such as collagen, muscle, and fibrin. The method utilizes a combination of three dyes - hematoxylin, acid fuchsin, and aniline blue - to stain the sample. This allows for the visualization of the different tissue components within a sample.
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Lab products found in correlation
4 protocols using masson s trichrome
1
Morphometric Analysis of Vascular Proliferation
2
Histological Evaluation of Implant Integration
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Fragments of the implants including surrounding host tissue were obtained after euthanasia. Specimens were fixed in F-13 solution, embedded in paraffin, and sliced into 5-μm sections. Paraffin sections were deparaffinized in xylol and a graded alcohol series, hydrated, stained with Masson’s trichrome and examined using a light microscope (Carl Zeiss, Oberkochen, Germany).
3
Calvarial Tissue Isolation and Analysis
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At four and 8 weeks,
calvarial were isolated, fixed in 10% paraformaldehyde (PFA)/phosphate-buffered
saline (PBS) for 48 h, and decalcified with 14% ethylenediaminetetraacetic
acid (EDTA) for 14 days. In the next step, after paraffin embedding,
serial tissue sections of 5 μm thickness were cut from the defect
site, stained with H&E, Alizarin Red, and Masson’s trichrome
(Asia Pajhohesh), and analyzed using a light microscope (Carl Zeiss
Inc., Germany).61 (link)
calvarial were isolated, fixed in 10% paraformaldehyde (PFA)/phosphate-buffered
saline (PBS) for 48 h, and decalcified with 14% ethylenediaminetetraacetic
acid (EDTA) for 14 days. In the next step, after paraffin embedding,
serial tissue sections of 5 μm thickness were cut from the defect
site, stained with H&E, Alizarin Red, and Masson’s trichrome
(Asia Pajhohesh), and analyzed using a light microscope (Carl Zeiss
Inc., Germany).61 (link)
4
Tissue Histological Analysis
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Three biopsies of each reconstructed tissue combination were fixed with HistoChoice’s solution and embedded in paraffin wax. Five-micrometer-thick sections were cut and stained with Masson’s Trichrome (Zeiss, Axio Imager, North York, ON, Canada).
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