1 1 diphenyl 2 picrylhydrazyl dpph

Manufactured by Solarbio

Sourced in China

1,1-diphenyl-2-picrylhydrazyl (DPPH) is a stable free radical used in colorimetric assays to assess the free radical scavenging capacity of various samples.

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Lab products found in correlation

4 protocols using 1 1 diphenyl 2 picrylhydrazyl dpph

Antioxidant Capacity Evaluation of OVA-Alginate Composites

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Ovalbumin (OVA, albumin from chicken egg white, nitrogen content ≥ 12.5%, total protein on a dry basis ≥ 80%, relative molecular mass less than 1000 ≥ 80%, pI: 4.43–4.66; purity ≥ 80%), sodium alginate [SA, viscosity, 10 g L⁻¹, 20 °C, (Pa s) ≥ 0.02], LB broth, LB nutrient agar, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and water-soluble vitamin E Trolox were purchased from Solarbio Technology Co., Ltd. (Beijing, China). Kaempferol (KAE, a polyphenol from brown algae, purity ≥ 97%), tannic acid (TA), potassium persulfate, potassium ferricyanide, and iron(iii)chloride anhydrous were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). The Folin-phenol reagent was purchased from Bomei Biotechnology Co., Ltd. (Hefei, China). All other chemicals used were analytical grade, and purchased from Sinopharm Chemical Glass Co., Ltd. (Jinzhou, China).

Tao X., Shi H., Cao A, & Cai L. (2022). Understanding of physicochemical properties and antioxidant activity of ovalbumin–sodium alginate composite nanoparticle-encapsulated kaempferol/tannin acid. RSC Advances, 12(28), 18115-18126.

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Ultrahigh Performance Liquid Chromatography for Flavonoid Analysis

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Methanol and acetonitrile used in ultrahigh performance liquid chromatography (UHPLC) were purchased from OmniGene LLC (Morrisville, NC, USA). Analytical flavonoid standards, i.e., catechin, myricetin, epicatechin, rutin, quercetin, liquiritigenin, cyanidin-3-O-glucoside, gallic acid, Trolox, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), quercetin-7-O-β-D-glucopyranoside, and 2,4,6-tri-(2-pyridyl)-1,3,5-triazine (TPTZ) were purchased from Solarbio Technology Co. Ltd. (Beijing, China).

Fu H., Qiao Y., Wang P., Mu X., Zhang J., Fu B, & Du J. (2021). Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge. PLoS ONE, 16(5), e0251300.

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Antioxidant Capacity of Tibetan Mushroom

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Dried fruiting bodies of T. mongolicum Imai were obtained from Zhangjiakou, Hebei Province, China. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China). The total antioxidant capacity assay kit for the ABTS method was purchased from Beyotime Co., Ltd. (Shanghai, China). Monosaccharide standards (mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, N-acetyl-D-glucosamine, N-acetyl-D- galactosamine, glucose, galactose, xylose, arabinose, and fucose) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). All the reagents and chemicals used in this study were analytical or HPLC grade.

Zhang N., Yang B., Mao K., Liu Y., Chitrakar B., Wang X, & Sang Y. (2022). Comparison of structural characteristics and bioactivity of Tricholoma mongolicum Imai polysaccharides from five extraction methods. Frontiers in Nutrition, 9, 962584.

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Soybean Protein and Wheat Gluten Characterization

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Soybean protein concentrate (SPC) was purchased from Hao Xiang (Guangzhou, China), wheat gluten (WG) was purchased from Wanbang (Henan, China). SPC contains approximately 75.3 % protein, 8.5 % moisture, 4.4 % ash, 14.9 % carbohydrate, and 2.4 % fat. WG contains around 81.3 % protein, 3.7 % moisture, 2.1 % ash, 12.2 % carbohydrate, and 1.1 % fat. Neutrase (8 × 10⁵ U/g) from Bacillus amyloliquefaciens was kindly provided by Novozymes (Bagsvaerd, Denmark). Fresh chicken breasts were purchased from a local supermarket in Harbin, China, and stored at 4 °C before use. 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) were both purchased from Solarbio (Beijing, China). Throughout the study, deionized (DI) water was used.

Zhang X., Zhao Y., Zhang T., Zhang Y., Jiang L, & Sui X. (2023). Potential of hydrolyzed wheat protein in soy-based meat analogues: Rheological, textural and functional properties. Food Chemistry: X, 20, 100921.

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