Penicillin G / streptomycin sulfate solution and powder to prepare Dulbecco’s modified Eagles medium (DMEM with 25 mM glucose; catalog number: 52100-021) were obtained from Thermo Fisher Scientific (Schwerte, Germany; RRID:SCR_008452). Glucose-free DMEM powder (catalog number: D5030), fetal calf serum (FCS), antimycin A, BAM15 and UK5099 were purchased from Sigma-Aldrich (Darmstadt, Germany; RRID:SCR_008988). AR-C155858 was purchased at Tocris Bioscience (Bristol, UK; RRID:SCR_003689). All enzymes used for the assays to determine pyruvate, lactate and glucose were purchased from Roche Diagnostics (Mannheim, Germany; RRID:SCR_001326). Other chemicals of the highest purity available were obtained from Merck (Darmstadt, Germany; RRID:SCR_001287), Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988), AppliChem (Darmstadt, Germany; RRID:SCR_005814) or Carl Roth (Karlsruhe, Germany; RRID:SCR_005711). Sterile cell culture materials and unsterile 96-well plates were from Sarstedt (Nümbrecht, Germany).
Penicillin g streptomycin sulfate solution
Manufactured by Thermo Fisher Scientific
Sourced in Germany
Penicillin G/streptomycin sulfate solution is a sterile, liquid culture medium additive used to prevent bacterial contamination in cell culture applications. It contains the antibiotics penicillin G and streptomycin sulfate at specific concentrations.
Automatically generated - may contain errors
Lab products found in correlation
Sterile cell culture material, by Sarstedt (4 mentions)
Bam15, by Merck Group (2 mentions)
Dulbecco s modified eagles medium dmem with 25 mm glucose, by Thermo Fisher Scientific (2 mentions)
Unsterile 96 well plates, by Sarstedt (2 mentions)
Bovine serum albumin (bsa), by AppliChem (2 mentions)
Perchloric acid, by AppliChem (2 mentions)
Cell titer glo 2.0 atp assay kit, by Promega (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Antimycin a, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by AppliChem (2 mentions)
Uk5099, by Merck Group (2 mentions)
Ar c155858, by Bio-Techne (1 mentions)
Oligomycin, by Merck Group (1 mentions)
Wst 1, by Dojindo Laboratories (1 mentions)
Etomoxir, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Rotenone, by Merck Group (1 mentions)
2 deoxy d glucose 2 dg, by Merck Group (1 mentions)
Creatine kinase, by Merck Group (1 mentions)
Black microtiter plates, by Sarstedt (1 mentions)
4 protocols using penicillin g streptomycin sulfate solution
1
Preparation of DMEM with additives
2
Metabolic Inhibitor Procurement and Usage
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2-Deoxy-d-glucose (2DG), antimycin A, BAM15, fetal calf serum (FCS), oligomycin and rotenone were purchased from Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988). Bovine serum albumin, dimethyl sulfoxide (DMSO), perchloric acid, NAD + , NADH and NADP + were from AppliChem (Darmstadt, Germany; RRID:SCR_005814). β-lapachone was from Abcam (Berlin, Germany; RRID:SCR_012931) and WST1 from Dojindo (Munich, Germany). Etomoxir and UK5099 were obtained from Merck (Darmstadt, Germany; RRID:SCR_001287). Dulbecco's modified Eagles medium (DMEM) and penicillin G/streptomycin sulfate solution were obtained from Thermo Fisher Scientific (Schwerte, Germany; RRID:SCR_008452). The Cell Titer Glo® 2.0 ATP Assay Kit was obtained from Promega (Walldorf, Germany; RRID:SCR_006724). ATP and the enzymes used for assays to quantify lactate, glucose, 2DG and 2DG6P were purchased from Roche Diagnostics (Mannheim, Germany; RRID:SCR_001326). All other basal chemicals were obtained from Carl Roth (Karlsruhe, Germany; RRID:SCR_005711), Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988), Riedel-de Haën (Seelze, Germany) or Fluka (Buchs, Switzerland). Sterile cell culture materials as well as transparent and black microtiter plates were obtained from Sarstedt (Nümbrecht, Germany).
3
ATP Quantification in Cell Cultures
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Sterile cell culture materials, unsterile 96-well plates and black microtiter plates were purchased from Sarstedt (Nümbrecht, Germany). Fetal calf serum (FCS), adenylate kinase, AMP, antimycin A, creatine, creatine kinase and 2-deoxyglucose (2DG) were obtained from Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988). ADP, Dulbecco’s modified Eagles medium (DMEM with 25 mM glucose, catalog number 52100-021) and penicillin G/streptomycin sulfate solution were from Thermo Fisher Scientific (Schwerte, Germany). The Cell Titer Glo® 2.0 ATP Assay Kit was purchased from Promega (Walldorf, Germany; RRID:SCR_006724). Bovine serum albumin, dimethyl sulfoxide (DMSO) and perchloric acid were from AppliChem (Darmstadt, Germany; RRID:SRC_005814). ATP and pyruvate kinase were purchased from Roche Diagnostics (Mannheim, Germany; RRID:SCR_001326). All other basal chemicals were obtained from Sigma-Aldrich (Steinheim, Germany), Roth (Karlsruhe, Germany), Riedel-de Haën (Seelze, Germany) or Fluka (Buchs, Switzerland).
4
Optimizing Cell Metabolism Assays
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Fetal calf serum (FCS), antimycin A (A8674), and BAM-15 (SML1760) were purchased from Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988). Etomoxir (HY-50202) and UK5099 (HY-15475) were obtained from Merck (Darmstadt, Germany). Dulbecco’s modified Eagles medium (DMEM) and penicillin G/streptomycin sulfate solution were from Thermo Fisher Scientific (Schwerte, Germany). Acetate, bovine albumin, dimethyl sulfoxide (DMSO), perchloric acid, NAD+, NADH and NADP+ were from AppliChem (Darmstadt, Germany; RRID:SRC_005814). The Cell Titer Glo® 2.0 ATP Assay Kit (G9241) was from Promega (Walldorf, Germany; RRID:SCR_006724). ATP and the enzymes used for the lactate and glucose assays were purchased at Roche Diagnostics (Mannheim, Germany; RRID:SCR_001326). All other basal chemicals were obtained from Sigma-Aldrich (Steinheim, Germany), Roth (Karlsruhe, Germany), Riedel-de Haën (Seelze, Germany) or Fluka (Buchs, Switzerland). Sterile cell culture materials as well as unsterile 96-well plates and black microtiter plates were obtained from Sarstedt (Nümbrecht, Germany).
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