Nuclear Magnetic Resonance (NMR) analysis was carried out to provide detailed structural information about the EPS [34 (link)]. The NMR analyses were performed on a Bruker 600 MHz equipped with a cryogenic probe, and spectra were recorded at 298 K. Acetone was used as internal standard (1 H 2.225 ppm, 13 C 31.45 ppm) and 2D spectra (1 H–1 H DQF-COSY, 1 H–1 H NOESY, 1 H–1 H TOCSY, 1–13 C HSQC and 1–13 C HMBC) were acquired by using Bruker software (TopSpin 2.0). 31P NMR experiments were carried out with a Bruker DRX-400 spectrometer; aqueous 85% phosphoric acid was used as an external reference (δ = 0.00 ppm). Homonuclear experiments were recorded using 512 FIDs of 2048 complex with 32 scans per FID; mixing times of 100 and 200 ms were used for TOCSY and NOESY spectra acquisition, respectively. HSQC and HMBC spectra were acquired with 512 FIDs of 2048 complex points, accumulating 40 and 80 scans, respectively. Spectra were processed and analyzed using a Bruker TopSpin 3 program.
Topspin 3 program
Manufactured by Bruker
TopSpin 3 is a software program developed by Bruker for the acquisition and processing of Nuclear Magnetic Resonance (NMR) data. The core function of TopSpin 3 is to provide a comprehensive suite of tools for NMR data management, analysis, and visualization.
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Lab products found in correlation
2 protocols using topspin 3 program
1
NMR Analysis of Extracellular Polysaccharides
2
NMR Analysis of Purified Polysaccharides
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NMR analyses were performed on a Bruker 600 MHz equipped with a cryogenic probe and spectra were recorded at 32°C for the purified CPS and at 45°C for dCPS. Acetone was used as internal standard (1H 2.225 ppm, 13C 31.45 ppm) and 2D spectra (DQF-COSY, TOCSY, NOESY, gHSQC and gHMBC) were acquired by using Bruker software (TopSpin 2.0). Homonuclear experiments were recorded using 512 FIDs of 2048 complex with 32 scans per FID, mixing times of 100 and 200 ms were used for TOCSY and NOESY spectra, respectively. HSQC and HMBC spectra were acquired with 512 FIDs of 2048 complex point, accumulating 40 and 80 scans, respectively. Spectra were processed and analyzed using a Bruker TopSpin 3 program.
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