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Indocyanine green icg

Sourced in United States, China

Indocyanine green (ICG) is a fluorescent dye used in various medical and research applications. It is a water-soluble anionic tricarbocyanine dye that exhibits near-infrared fluorescence when exposed to light in the appropriate wavelength range. ICG is commonly used as a contrast agent for various imaging techniques, such as angiography, fluorescence-guided surgery, and tissue perfusion assessment.

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3 protocols using indocyanine green icg

1

Copper-based Nanoparticle Synthesis and Evaluation

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Cetyltrimethylammonium chloride (CTAC), tetraethyl orthosilicate, 2,7-dichlorofluorescein diacetate (DCFH-DA), dopamine hydrochloride, Hoechst 33258, and ethanol butanol were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Dimethyl thiazole blue (MTT) was purchased from Beijing Solarbio Corporation (Beijing, China). DSF, fluorescein isothiocyanate (FITC), and indocyanine green (ICG) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Cupric nitrate trihydrate (Cu(NO3)2·3H2O), ammonia solution (NH3·H2O), hydrochloric acid (HCl), dimethyl sulfoxide, hydrogen peroxide (H2O2), triethanolamine, and phosphate buffer saline (PBS) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The L929 and HCT116 cells were supplied by the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Acridine orange/ethidium bromide reagent was purchased from Yuan Ye Biological Co., Ltd. (Shanghai, China). Deionized water was used in this study, and all the compounds utilized in the experiments were of analytical grade, including the aforementioned compounds.
All experiments involving animals were approved by the Animal Ethics Committee of Zhejiang Ocean University (approval No. 2021055). Male BALB/c nude mice (18–22 g) were provided by the Ziyuan Laboratory Animals (Hangzhou, China).
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2

Fluorescent Nanoparticle Uptake Assay

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Cetyltrimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), 3-aminopropyltriethoxysilane (APTES), and fluorescein isothiocyanate (FITC) were procured from Sigma-Aldrich (Saint Louis, MO, USA); lithocholic acid (LCA), N,N-dimethylformamide (DMF), chlorpromazine, amiloride, indocyanine green (ICG, 95%), ethynodiol diacetate, and silicon standard solution were sourced from Macklin (Shanghai, China); anhydrous ethanol, 25% ammonia solution, and paclitaxel standard (≥99%) were obtained from Aladdin (Shanghai, China); paclitaxel (PTX, 99%) was acquired from HEOWNS (Tianjin, China); RPMI-1640 medium, penicillin-streptomycin solution, phosphate-buffered saline (PBS), 0.25% trypsin (Trypsin-EDTA), and fetal bovine serum were purchased from Gibco (Saint Louis, MO, USA); the CCK-8 assay kit, 4′,6-diamidino-2-phenylindole (DAPI), and various organelle red probes were sourced from Beyotime (Shanghai, China); and methyl-β-cyclodextrin was obtained from Yuanye (Shanghai, China).
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3

Multimodal Imaging and Molecular Regulation

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3,3′-dioctadecyloxacarbocyanine perchlorate
(DiO), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate
(DiI), 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4-Chlorobenzenesulfonate
Salt(DiD), ROS flow kit, and Western blot reagents were purchased
from Beyotime Biotechnology (China). The cytokines used in this study
(TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)),
lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress
(MCE, China). Indocyanine green (ICG) was obtained from Macklin Inc.
(China). The apoptosis flow kit and cell counting kit-8 (CCK-8) cell
viability assay kit were purchased from Vazyme Biotech Co., Ltd. (China).
The J774A.1 cell line was purchased from Procell Life Science &
Technology Co., Ltd. (China). Lipofectamine 3000, PBS, media, serum,
and ELISA kits required for cell culture were purchased from Thermo
Fisher Scientific Inc. (Waltham, MA, USA). The PCR-related reagents
were purchased from Yeasen Biotechnology Co., Ltd. (China). The animals
needed for the experiment were purchased from the Laboratory Animal
Center of Huazhong University of Science and Technology.
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