Shh silencer select validated sirna

Manufactured by Thermo Fisher Scientific

Sourced in United States

Shh Silencer Select Validated siRNA is a laboratory tool designed for gene silencing experiments. It functions as a short interfering RNA (siRNA) molecule that can be used to suppress the expression of the Sonic Hedgehog (Shh) gene in cultured cells.

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Lab products found in correlation

Lipofactamine 2000, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Silencer Select siRNAs (565 citations) Silencer Select (341 citations) SiRNAs (258 citations) Silencer® Select Validated siRNA (54 citations) Silencer siRNA (37 citations) Validated select siRNA (4 citations)

2 protocols using shh silencer select validated sirna

Transient Silencing and Overexpression of Shh

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For transiently silencing Shh, the Shh Silencer Select Validated siRNA was purchased from Life Technologies, United States (Catalog #AM16708). For overexpression of Shh, human Shh full-length cDNA was cloned into the pCMV expression vector. The Shh siRNA and pCMV-Shh (2 μg/μL) was transiently transfected into TE-1 or KYSE-510 cells by Lipofactamine 2000 (Invitrogen) according to the manufacturer’s instructions. Transfection efficiency was verified by western blotting.

Cui W., Fang T., Duan Z., Xiang D., Wang Y., Zhang M., Zhai F., Cui X, & Yang L. (2020). Dihydroartemisinin Sensitizes Esophageal Squamous Cell Carcinoma to Cisplatin by Inhibiting Sonic Hedgehog Signaling. Frontiers in Cell and Developmental Biology, 8, 596788.

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Modulation of Sonic Hedgehog Signaling

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For transiently silencing Shh, the Shh Silencer Select Validated siRNA was purchased from Life Technologies, USA (Catalog #AM16708). For overexpression of Shh, human Shh full-length cDNA was cloned into the pCMV expression vector. The Shh siRNA and pCMV-Shh (2 μg/μL) was transiently transfected into TE-1 or KYSE-510 cells by Lipofactamine 2000 (Invitrogen) according to the manufacturer's instructions. Transfection e ciency was veri ed by western blotting.

Cui W., Fang T., Duan Z., Xiang D., Wang Y., Zhang M., Zhai F., Cui X., & Yang L. (2020). Dihydroartemisinin Sensitizes Esophageal Squamous Cell Carcinoma to Cisplatin by Inhibiting Sonic Hedgehog Signaling.

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