H e staining kit

Manufactured by Meilun

Sourced in China

The H&E staining kit is a laboratory equipment used to stain tissue samples for microscopic analysis. It contains the necessary reagents and protocols to perform the hematoxylin and eosin (H&E) staining technique, which is a widely used method in histology and pathology.

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Lab products found in correlation

Ti s inverted phase contrast microscope, by Nikon (1 mentions)

3 protocols using h e staining kit

Histological Analysis of Mouse Aorta

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The mouse thoracic aortas were collected and cut into 8 μm frozen sections. A H&E staining kit (MeilunBio, Dalian, China) was used to stain the tissue according to the manufacturer’s instructions. Finally, the tissue condition was observed using an optical microscope. ImageJ was used to measure the media thickness and vessel lumen diameter.

Zong T., Yang Y., Lin X., Jiang S., Zhao H., Liu M., Meng Y., Li Y., Zhao L., Tang G., Gong K., Wang Z, & Yu T. (2021). 5′-tiRNA-Cys-GCA regulates VSMC proliferation and phenotypic transition by targeting STAT4 in aortic dissection. Molecular Therapy. Nucleic Acids, 26, 295-306.

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Carotid Artery Morphology Analysis

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A continuous cross section of the carotid artery was obtained from the injured area, and sections were collected from each vessel for morphological analysis. The H&E staining was carried out according to the instructions of H&E staining kit (Meilunbio, Dalian, China), and the images were taken by Nikon Ti–S inverted phase contrast microscope (Olympus，Tokyo，Japan), followed by ruler addition by Image J software.

Xue Q., Yu T., Wang Z., Fu X., Li X., Zou L., Li M., Cho J.Y, & Yang Y. (2022). Protective effect and mechanism of ginsenoside Rg2 on atherosclerosis. Journal of Ginseng Research, 47(2), 237-245.

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Carotid Artery Morphometric Analysis

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Serial cross-sections of carotid arteries (8 μm) were obtained at the injured area, and six sections from each vessel were collected for morphometric analysis. H&E staining was performed following the instructions of the H&E staining kit (Meilunbio, Dalian, China), and images were captured by a Nikon Ti-S inverted phase-contrast microscope. For the quantitative analysis, areas of intima and media were measured using ImageJ software.

He X., Lian Z., Yang Y., Wang Z., Fu X., Liu Y., Li M., Tian J., Yu T, & Xin H. (2020). Long Non-coding RNA PEBP1P2 Suppresses Proliferative VSMCs Phenotypic Switching and Proliferation in Atherosclerosis. Molecular Therapy. Nucleic Acids, 22, 84-98.

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