Fitc antibody labeling kit

Human haptoglobin (from pooled human plasma, a mixture of 1-1, 2-1, and 2-2 phenotype, catalog 3536), REDExtract-N-Amp Tissue PCR kit (catalog XNATS), Triton X-114, imidazole, monoclonal anti-human haptoglobin antibodies (catalog H6395), nonimmune mouse IgG, Drabkin’s reagents (catalog D5941), human M-CSF, DAPI, and dexamethasone (catalog D2915) were purchased from Sigma-Aldrich. TACS MTT Cell proliferation assay kit was from Trevigen. Trizol reagent was from Invitrogen. The RevertAid First Strand cDNA Synthesis Kit was from Fermentas. SYBR Premix Ex Taq II was from Takara Bio Inc. Thioglycollate medium was purchased from BD Biosciences. Primers for quantitative PCR (qPCR), trypsin-EDTA, and carbenicillin were from Invitrogen. Protein A/G agarose and isopropyl-D-thiogalacto-pyranoside (IPTG) were purchased from Pierce. NHS-activated Sepharose 4 fast flow beads were obtained from GE Healthcare (catalog 17-0906-01). E. coli strain DH5α was from Novagen. Recombinant human CD163 protein, CD163 expression plasmid (SC117495, NM_004244.3), and MegaTran 1.0 transfection reagent were purchased from Origene. Antibodies for human HO-1 (catalog ab52946) and CD163 (catalog MCA1853) were from Abcam and Serotec, respectively. Anti–human CD163-PE antibody was from BioLegend. FITC antibody labeling kit was from Thermo Scientific. Dynasore was from Tocris Bioscience.

Human haptoglobin (from pooled human plasma, a mixture of Hp1-1, 2-1, 2-2, Cat #3536), triton X-114, imidazole, human macrophage-colony stimulating factor (M-CSF), acetaminophen and dexamethasone (Cat # D2915) were purchased from Sigma (St. Louis, MO). NHS-activated sepharose 4 fast flow beads were obtained from GE Healthcare (Cat #17-0906-01, Uppsala, Sweden). Trizol reagent was from Life Technologies (Carlsbad, CA). The RevertAid™ First Strand cDNA Synthesis Kit was from Fermentas (Vilnius, Lithuania). SYBR Premix Ex Taq™ II was from TaKaRa Bio Inco (Otsu, Japan). Thioglycollate medium was purchased from Becton Dickinson Co., (Sparks, MD). Primers for QPCR, trypsin-EDTA and carbenicillin were from Invitrogen Inc., (Carlsbad, CA). Isopropyl-D-thiogalactopyranoside (IPTG) was purchased from Pierce (Rockford, IL). Recombinant human CD163 protein, CD163 expression plasmid (SC117495, NM_004244.3) and MegaTran 1.0 transfection reagent were purchased from Origene (Rockville, MD). Antibodies for human heme oxigenase-1 (HO-1, Cat # ab52946) and CD163 (Cat # MCA1853) were from ABcam (Cambridge, MA) and Serotec (Raleigh, NC) respectively. Anti-human CD163-PE and anti-human CD80-PE antibodies were from BioLegend (San Diego, CA). FITC antibody labeling kit is from Thermo Scientific (Rockford, IL). Alanine transaminase (ALT) assay kits were from BIOO Scientific Corp., (Austin, TX).