S methylisothiourea hemisulfate salt

The catecholamine epinephrine hydrochloride (EPI), the NOS inhibitors S-methylisothiourea hemisulfate salt (SMIS) and aminoguanidine hemisulfate salt (AGH) as well as the NO donor sodium nitroprusside dihydrate (SNP) were purchased from Sigma-Aldrich. The irreversible inhibitor of soluble guanylyl cyclase 1H-[1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ) and the NO donor 3-morpholinosydnonimine chloride (SIN-1) were obtained from AdipoGen Life Sciences and the NOS inhibitors L-NG-nitroarginine methyl ester (L-NAME) hydrochloride, L-NG-nitroarginine (L-NNA) and 7-nitroindazole (7-NI) were purchased from Cayman chemicals. Additional chemicals were Levodopa (L-DOPA), obtained from Santa Cruz Biotechnology, as well as (+)-MK 801 maleate (MK-801) and Ifenprodil (+)-tartrate salt (ifenprodil), both obtained from Selleckchem. Stock solutions at 10^− 1 M for SMIS, AGH and SNP or at 10^− 2 M for remaining compounds were either prepared with autoclaved Milli-Q dH₂O or with DMSO (Sigma-Aldrich) for 7-NI and ODQ. Working solutions (10x concentrate of final concentration of treatment) for each compound were prepared prior to experiments with 1 μm filtered fresh seawater (FSW).