The cellular uptake and transport of the IPC-DNVs and IPC-DNV-TSG were compared using a widely established buccal absorption model with TR146 cells. The TR146 cell line was provided by Guangzhou Biotechnology Company and cultured as previously described [26 (link)]. Briefly, TR146 cells in the exponential growth phase were washed once with PBS, digested with 0.25% trypsin, cultured in 1640 medium containing 10% fetal bovine serum, and suspended to a final concentration of 4 × 105 cells/mL. The cell suspension was seeded in the upper chamber of a 12-well transwell cell culture chamber (pore size: 0.4 μm; Corning Inc., New York, NY, USA) at a cell density of 8 × 104 cells/cm2, and 1.5 mL of medium was placed in the bottom well. Using the liquid-cover culture method, the culture medium was changed every 2–3 days, and cells were cultured for 28–32 days.
12 well transwell cell culture chamber
Manufactured by Corning
Sourced in United States
The 12-well transwell cell culture chamber is a laboratory equipment used for cell culture experiments. It consists of a multi-well plate with a semi-permeable membrane insert that separates the upper and lower compartments, allowing for the study of cell migration, invasion, and other cellular processes in a controlled environment.
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Lab products found in correlation
2 protocols using 12 well transwell cell culture chamber
1
Buccal Absorption Model for Cellular Uptake
2
Caco-2 Cell Culture Protocol
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Caco-2 cells were purchased from the American Type Culture Collection (Rockville, MD, USA). In this study, Caco-2 cells were used
between passages 35 and 45. The growth medium consisted of Dulbecco’s modified Eagle’s medium with 10% FBS, 1% nonessential amino
acids, and antibiotics (100 units/ml penicillin, 100 μg/ml streptomycin, and 50
μg/ml gentamycin). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere
in 75-cm2 tissue culture flasks to approximately 80% confluence and seeded into a 12-well Transwell cell culture
chamber (0.4-μm pore size, 12-mm diameter) (Corning Inc., Tewksbury, MA, USA) at a density of 5 × 104cells/cm2. After 14 days of culture, transepithelial resistance (TER) was measured using a Millicell-ERS instrument
with Ag/AgCl electrodes (EMD Millipore Corporation, Billerica, MA, USA). Caco-2 cell monolayers were used when their TER values
were >300 ohm•cm2. Each well was placed in a cluster plate with an external medium (basolateral side, 1.5
ml) and an internal medium (apical side, 0.5 ml). The cell monolayers were fed fresh medium
every 24 hr.
between passages 35 and 45. The growth medium consisted of Dulbecco’s modified Eagle’s medium with 10% FBS, 1% nonessential amino
acids, and antibiotics (100 units/ml penicillin, 100 μg/ml streptomycin, and 50
μg/ml gentamycin). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere
in 75-cm2 tissue culture flasks to approximately 80% confluence and seeded into a 12-well Transwell cell culture
chamber (0.4-μm pore size, 12-mm diameter) (Corning Inc., Tewksbury, MA, USA) at a density of 5 × 104cells/cm2. After 14 days of culture, transepithelial resistance (TER) was measured using a Millicell-ERS instrument
with Ag/AgCl electrodes (EMD Millipore Corporation, Billerica, MA, USA). Caco-2 cell monolayers were used when their TER values
were >300 ohm•cm2. Each well was placed in a cluster plate with an external medium (basolateral side, 1.5
ml) and an internal medium (apical side, 0.5 ml). The cell monolayers were fed fresh medium
every 24 hr.
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