Absorption spectra (Lambda 35 UV/Vis System, PerkinElmer) and emission spectra (FluoTime 300, PicoQuant) were recorded in HEPES buffer (100 mM, pH 7.4) and deionized water (purified by Purelab Option-Q equipped with bio-filter, ELGA LabWater) for Tb and Cy5.5 samples, respectively.
Lambda 35 uv vis system
Manufactured by PerkinElmer
The Lambda 35 UV/Vis System is a compact, high-performance double-beam spectrophotometer designed for a wide range of UV/Vis applications. It features a wavelength range of 190 to 1100 nm, variable slit widths, and a photometric range of -4 to 4 Abs. The system provides accurate and reliable absorbance measurements for various sample types.
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3 protocols using lambda 35 uv vis system
1
Spectroscopic Analysis of Tb and Cy5.5
2
Photophysical Characterization of Luminescent Probes
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Absorption spectra were recorded by a Lambda 35 UV/Vis System (PerkinElmer). PL decay curves were obtained by an EI fluorescence plate reader (Edinburgh Instruments) by the use of 4000 detection bins with 2 μs integration time and excitation at 337.1 nm with a nitrogen laser (20 Hz, VSL 337 ND, Spectra-Physics). Optical bandpass filters (Semrock) were (494 ± 10) nm for the Tb detection, (660 ± 7), (607 ± 5) and (707 ± 13) nm for the QD650, QD605 and QD705 detection channels, respectively.
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Absorption spectra were recorded by a Lambda 35 UV/Vis System (PerkinElmer). PL decay curves were obtained by an EI fluorescence plate reader (Edinburgh Instruments) by the use of 4000 detection bins with 2 μs integration time and excitation at 337.1 nm with a nitrogen laser (20 Hz, VSL 337 ND, Spectra-Physics). Optical bandpass filters (Semrock) were (494 ± 10) nm for the Tb detection, (660 ± 7), (607 ± 5) and (707 ± 13) nm for the QD650, QD605 and QD705 detection channels, respectively.
3
Copper(II) Pyridine-2,4-dicarboxylate Synthesis
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All chemicals and solvents used for the synthesis were of AR grade. Triethylamine, copper(II) nitrate trihydrate, were obtained from E. Merck, India. Pyridine-2,4dicarboxylic acid and 2-picolylamine (2-aminomethylpyridine) were purchased from Aldrich Chemical Co. All chemicals were used without further purification. Elemental analyses (carbon, hydrogen and nitrogen) were performed with a Perkin Elmer 2400 II Elemental Analyser. Copper(II) content of 1 has been estimated quantitatively by standard iodometric procedure. The Fourier transform infrared spectrum was recorded on a Perkin Elmer RX-I FT-IR spectrophotometer, with solid KBr disc, in the range 4000-400 cm -1 . Solid state UV-Vis spectrum of the title complex was recorded on a Perkin Elmer Lambda 35 UV-Vis system in the range 1100-200 nm. TG analysis was performed with a Perkin-Elmer (Singapore) Pyris Diamond TGA unit. Thermal study was performed at the temperature range 35-800 °C by maintaining the heating rate at 5 °C min -1 in a stream of nitrogen flowing at the rate of 50 mL min -1 with the sample in a platinum crucible. Powder X-ray diffraction was performed on a Bruker D8 instrument with Cu-Kα radiation. (2,4-
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