Multi species glp 1 total elisa

At the Sahlgrenska University Hospital, blood glucose was measured using StatStrip according to the manufacturer’s instructions (Nova Biomedical, Waltham, MA, USA). At Ersta Hospital, blood glucose was measured using the YSI Model 2300 Stat Plus glucose analyzer according to the manufacturer’s instructions (Yellow Springs Instruments, OH, USA). Insulin was measured by an electrochemiluminescence immunoassay “ECLIA” using a Cobas e immunoassay analyzer according to the manufacturer’s instructions (Roche Diagnostics, Dublin, Ireland). The intra-assay variation of the insulin measurement was 1.1–1.4% (CV) and the inter-assay variation 3.5–3.7% (CV). The cross-reactivity with IGF-1 was 0.04%. GLP-1 and GIP were measured using ELISAs according to the manufacturer’s instructions (Merck Millipore, Solna, Sweden; Human total GIP ELISA, product number EZHGIP-54K, and Multi Species GLP-1 Total ELISA, product number EZGLP1T-36K). The intra-assay variation for GLP-1 was 1–2% (CV) and the inter-assay variation < 12% (CV). For GIP, the intra-assay variation was 3–8.8% (CV) and the inter-assay variation 1.8–6.1% (CV).

Blood glucose was measured using StatStrips according to the manufacturer’s instructions (Nova Biomedical, Waltham, MA). Fasting serum insulin was measured by an electrochemiluminescence immunoassay, “eclia”, using a Cobas immunoassay analyzer according to the manufacturer’s instructions (Roche Diagnostics, Rotkreutz, Switzerland). Insulin, GLP-1 and GIP in serum samples taken after various times during the MMTs (0, 15, 30, 45, 60, 90 and 120 min for insulin, and 0, 30, 60 and 120 min for GLP-1 and GIP) were quantified using ELISA techniques according to the manufacturer’s instructions. Kits used were Human Insulin ELISA, Multi Species GLP-1 Total ELISA and Human GIP (total) ELISA (Merck Millipore, Darmstadt, Germany). HOMA-IR was calculated using the formula [fasting glucose (mmol/L) × fasting insulin (mU/L)/22.5].