Log In Sign up
The largest database of trusted experimental protocols

Automated spectrophotometer

Manufactured by Agilent Technologies
Visit Supplier
Sourced in United States

The Automated Spectrophotometer is a laboratory instrument designed to measure the absorption or transmission of light by a sample. It automates the process of spectrophotometric analysis, providing accurate and reproducible measurements across a range of wavelengths.

Automatically generated - may contain errors

Lab products found in correlation

Tetramethylbenzidine substrate, by Thermo Fisher Scientific (1 mentions) Inspect f, by Thermo Fisher Scientific (1 mentions) Elisa assays, by R&D Systems (1 mentions)

5 protocols using automated spectrophotometer

1

Bacterial Growth Dynamics with tFNAs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The strains were grown from a single colony in BHI both until early log phase was achieved at an optical density at 595 nm (OD595) of 0.2–0.3. After adjusting OD to a common value, bacteria were cultured in the presence of different concentrations of tFNAs and ASOs-tFNAs in BHI medium in a cell culture plate. An automated spectrophotometer (BioTek, Winooski, VT, USA) was used to measure planktonic bacterial growth, and OD595 readings were taken over a period of 24 h every 2 h, with plate shaking every 30 min.
Zhang Y., Xie X., Ma W., Zhan Y., Mao C., Shao X, & Lin Y. (2020). Multi-targeted Antisense Oligonucleotide Delivery by a Framework Nucleic Acid for Inhibiting Biofilm Formation and Virulence. Nano-Micro Letters, 12, 74.
+ Open protocol
+ Expand
2

Evaluating tFNAs' Impact on E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols
In order to observe the effect of tFNAs on E. coli growth, the OD600nm of bacteria was observed after 24 h of co-culture with different concentrations of tFNAs (100–500 nM). The control group was the E. coli with no treatment. The E. coli were adjusted with an initial density of 5 × 105 CFU/mL to set up different experimental groups in the 96-well plates for the minimum inhibitory concentration (MIC) test. For detailed experimental procedures, please refer to supplementary document. The OD600nm values of each group was recorded from 0 to 24 h through an automated spectrophotometer (BioTek Instruments, USA) and was drawn as the growth curve. The OD600nm values of the erythromycin, ssDNA-Ery and tFNAs-Ery group were recorded at 24 h to compare the bacterial growth between groups. Sterile straws were used to absorb the diluted E. coli liquid from the 96 wells plate corresponding to drug concentration of 0.25 × MICEry and inoculate it on MHB agar plate into a 37 °C incubator for 24 h, for the enumeration by plate count.
Sun Y., Liu Y., Zhang B., Shi S., Zhang T., Zhao D., Tian T., Li Q, & Lin Y. (2021). Erythromycin loaded by tetrahedral framework nucleic acids are more antimicrobial sensitive against Escherichia coli (E. coli). Bioactive Materials, 6(8), 2281-2290.
+ Open protocol
+ Expand
3

Quantification of Autoantibody Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
96-well microplates were coated with 5 μg/ml of recombinant human proteins Chitinase 3-like 1 (CHI3L1), delta-like ligand 4 (DLL-4) (Sino Biological) or surfactant protein D (SFTPD) (R&D Systems), washed with PBS/0.1 % Tween®20, blocked with PBS/ 1% BSA and incubated with 1:100 diluted human sera or negative control (BSA only), followed by HRP-conjugated anti human IgM (1:5000) and tetramethylbenzidine substrate (Thermo Scientific, USA). The reaction was stopped with H2SO4 and the optical density (OD) was determined at 450nm using an automated spectrophotometer (BioTek). A standard curve was generated for each autoantibody utilizing commercially available antibodies and expressed as relative units (RU)/ml. Serum sample concentrations were calculated from the standard curves. Background OD levels based on BSA controls were subtracted from each experimental sample read.
Luo Y., Brigham D., Bednarek J., Torres R., Wang D., Ahmad S, & Mack C.L. (2021). Unique cholangiocyte-targeted IgM autoantibodies correlate with poor outcome in biliary atresia. Hepatology (Baltimore, Md.), 73(5), 1855-1867.
+ Open protocol
+ Expand
4

Evaluating Antimicrobial Effects on E. coli and MRSA

Check if the same lab product or an alternative is used in the 5 most similar protocols
E. coli was conducted using a 96-well plate, which was placed in a biochemical incubator (Thermo Scientific, USA) at 37 °C for 24 h. The minimum inhibitory concentration (MIC) of erythromycin for E. coli was recorded as MICEry, and that of ampicillin for MRSA was recorded as MICAmp. An automated spectrophotometer (BioTek Instruments, USA) was used to measure the OD600 value of the bacteria from 0 to 24 h, and the growth curve of each group was drawn. For evaluating the colony count, quantitative bacterial solutions from each group were transferred to Mueller–Hinton broth (MHB) agar plates for enumeration after incubation at 37 °C for 24 h. For morphological observation, the samples were prepared for scanning electron microscopy (FEI, INSPECTF, USA) to observe the changes in the number and spatial structure of bacteria treated by each group. The untreated bacteria were used as the control group. Each experiment was performed in triplicates.
Sun Y., Chen X., Shi S., Tian T., Liu Z., Luo E, & Lin Y. (2023). Tetrahedral Framework Nucleic Acids: A Novel Strategy for Antibiotic Treating Drug-Resistant Infections. Biomacromolecules, 24(2), 1052-1060.
+ Open protocol
+ Expand
5

Quantification of Neuroinflammatory Markers and Amyloid-Beta in Rodents

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The levels of IL-1β, IL-4, IL-6, IL-10, TNF-α, IFN-γ, in hippocampal homogenates, serum and CSF samples, and the levels of Aβ1-42 peptide in hippocampal homogenates and CSF were quantified using ELISA assays (R&D Systems, Minneapolis, USA or Invitrogen, Thermo Fisher Scientific, MA, USA), by evaluating the absorbance at 450 and 560 nm in an automated spectrophotometer (Bio-Tek, Winooski, VT, USA), as previously described (37 (link)). The Aβ1-42 peptide ELISA assay detects and quantifies mouse and rat Aβ1-42, both natural and synthetic. For the quantification of the different molecules, each animal was considered individually. No sample was held as a pool.
Díaz-Zúñiga J., More J., Melgar-Rodríguez S., Jiménez-Unión M., Villalobos-Orchard F., Muñoz-Manríquez C., Monasterio G., Valdés J.L., Vernal R, & Paula-Lima A. (2020). Alzheimer’s Disease-Like Pathology Triggered by Porphyromonas gingivalis in Wild Type Rats Is Serotype Dependent. Frontiers in Immunology, 11, 588036.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!