Iron 2 sulfate heptahydrate feso4 7h2o

SEM and EDS-SEM were performed using a JSM-5600LV SEM (JEOL, Tokyo, Japan) with an acceleration voltage of 5 to 10 kV. Samples were initially coated with a thin layer of Au/Pd using a Hummer 6.2 sputtering system (Anatech, Battle Creek, MI, USA) and then imaged. EDS was performed in the dark field, and both SEM and EDS-SEM were performed at high vacuum settings. Samples for cross-sectional analysis were first frozen by liquid nitrogen and then cut under an optical microscope. Iron(II) sulfate heptahydrate (FeSO₄.7H₂O) was purchased from Sigma-Aldrich and dispensed (100 ng) into 4X-core particles using a cellenONE picoliter dispenser. Nanoresolution x-ray CT reconstructs (resolution, 0.38 to 0.45 μm) were obtained at the Biotechnology Resource Center of Cornell University. NanoCT imaging was performed using a ZEISS/Xradia Versa 520 x-ray microscope. The three cross sections depicted in Fig. 1 for each particle were selected to be equidistant (50 to 60 μm apart). Reconstruction of nanoCT scans was performed with Avizo software (Thermo Fisher Scientific, USA). All 2D image analysis including porosity measurements was performed by ImageJ.

Cyclophosphamide was obtained from Pfizer International (NY, USA). Lactate dehydrogenase (LDH), total protein, cholesterol, and triacylglycerol kits were products of Randox Laboratories Limited (Admore, Crumlin, Co-Antrim, UK). Griess reagent kit was purchased from Cayman Chemical (Ann Arbor, Michigan, USA). Sodium nitroprusside (C₅FeN₆Na₂O), hydrogen peroxide (H₂O₂), butylated hydroxytoluene (BHT), vitamin C, 2- thiobarbituric acid (2, 6-dihydroxypyrimidine-2-thiol; TBA), dipotassium hydrogen phosphate (K₂HPO₄), potassium dihydrogen phosphate (KH₂PO₄), potassium chloride (KCl), reduced glutathione (GSH), oxidized glutathione (GSSG), 1-chloro-2, 4-dinitrobenzene (CDNB), Ellman’s reagent DTNB [5,5ˈ-dithiobis(2-nitrobenzoic acid)], pyrogallol, reduced nicotinamide adenine dinucleotide phosphate (NADPH), and xylenol tetrasodium (C₃₁H₂₈N₂Na₄O₁₃S), chloroform, isopropanol, triton X-100, ethanol, ammonium ferrothiocyanate, copper II nitrate (Cu(NO₃)₂.3H₂O), triethanolamine, oxalic acid bis(cyclohexylidenehydrazide), and ammonium hydroxide (NH₄OH) were procured from Aldrich Sigma Chemical Company (St. Louis, MO, USA). Iron (II) sulfate heptahydrate (FeSO₄.7H₂O) was imported from Merck KGaA, Darmstadt, Germany. Sulfuric acid was supplied by Ajax Finechem, Australia. All other chemicals and reagents were of analytical grade and were procured from British Drug Houses (Poole, UK).