Anti atp6ip2 ab40790

Left and right kidney tissue blocks were prepared and deparaffinized as described previously (12 (link)). Sections (n=4) were incubated overnight at 4°C with primary antibody directed against PRR monoclonal antibody (1:100 dilution; anti-ATP6IP2/ab40790; Abcam, Cambridge, MA, USA), collagen I (1:100 dilution; Abcam, Cambridge, MA, USA). On the following day, sections were incubated for 1hr with secondary antibody at room temperature. A negative control (IgG) was included by omitting the primary antibody. The immunostaining images were captured by light microscopy using a Q-imaging Micropublisher 5.0 RTV camera coupled to a Zeiss Axiophot microscopy (Carl Zeiss, Jena, Germany).

Antibodies to PRR (anti-ATP6IP2/ab40790, Abcam, Cambridge, MA, USA), TNF-α (Santa Cruz biotechnology, Inc., Santa Cruz, CA, USA), COX-2 (Novus bilogicals, Colorado, USA), NF-κB p65 (Abcam, Cambridge, MA, USA) and MCP-1 (Cell Signaling, USA) were used in the Western blot (n=4) as previously described (12 (link)). Protein expression was normalized to β-actin protein.