Serum samples were analyzed for calcium, inorganic phosphate, total protein and blood urea nitrogen (BUN), and also with a commercially available enzyme-linked immunosorbent assay (ELISA) kit for insulin (Mercodia, Uppsala, Sweden), bone specific alkaline phosphatase (BAP; Biomedical Technology Inc., Stoughton, MA, USA) and tartrate-resistant acid phosphatase-5b (TRACP-5b; Immunodiagnostic Systems Ltd., Boldon, UK).
Elisa kit for insulin
Manufactured by Mercodia
Sourced in Sweden
The ELISA kit for insulin is a laboratory equipment used to measure the concentration of insulin in a sample. It is a type of immunoassay that utilizes antibodies specific to insulin to detect and quantify the target analyte in the sample.
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Lab products found in correlation
2 protocols using elisa kit for insulin
1
Serum Biomarkers for Bone Health
2
Quantitative Insulin Assessment Protocol
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During the experiment, glucose meter and blood glucose test strips (TD-4207, EasiCheck, Taiwan) detected fasting blood glucose. A commercial enzyme-linked immunosorbent assay (ELISA) kit for insulin (catalog no. 10-1250-01, Mercodia, Sweden) was used for specimen detection. Briefly, sample (either serum or tissue homogenate) and standards were incubated in a 96-well plate with certain primary antibodies. The secondary antibodies, horseradish peroxidase-avidin, and chromogenic substrate 3,3’,5,5’-tetramethylbenzidine, were added and mixed, data read at OD of 450 nm. HOMA-IR index was calculated as fasting glucose (mmol/L)×fasting insulin (U/mL)/22.5.
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