Western blotting was performed using routine methods. Primary antibodies against the following proteins were used: mouse anti-GAPDH (1:5,000; Invitrogen MA5-15738), and rabbit anti-A1 AR (1:200; Alomone AAR-006). Secondary antibodies were used: IRDye 800CW-conjugated goat anti-rabbit IgG antibody (1:5,000; Li-Cor 926–32211), or IRDye® 680RD-conjugated goat anti-mouse IgG antibody (1:5,000; Li-Cor 926–68070). Protein bands were visualized using an Odyssey Infrared Imaging System (Li-Cor Biosciences). Details are provided in the Supplementary Methods.
Irdye 680rd conjugated goat anti mouse igg antibody
Manufactured by LI COR
The IRDye® 680RD-conjugated goat anti-mouse IgG antibody is a detection reagent designed for use in immunoassays and other applications. The antibody is conjugated with the IRDye® 680RD fluorescent dye, which enables near-infrared fluorescence detection.
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Lab products found in correlation
Odyssey infrared imaging system, by LI COR (2 mentions)
Irdye 800cw conjugated goat anti rabbit igg antibody, by LI COR (1 mentions)
Mouse anti gapdh, by Thermo Fisher Scientific (1 mentions)
Irdye 800cw conjugated goat anti rabbit igg, by LI COR (1 mentions)
Jnk1 2, by BD (1 mentions)
Flag m2, by Merck Group (1 mentions)
Clone b 5 1 2, by Merck Group (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using irdye 680rd conjugated goat anti mouse igg antibody
1
Western Blotting for Protein Detection
2
Protein Immunoblot Analysis of Cellular Signaling
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Extracts (20 to 50 µg of protein) were examined by protein immunoblot analysis by probing with antibodies to JUN (Cell Signaling Technology, Cat# 2315, RRID: AB_490780), pSer63-JUN (Cell Signaling Technology, Cat# 9261, RRID: AB_213016292), GAPDH (Santa Cruz Biotechnology, Cat# sc-25778, RRID: AB_10167668), JNK1/2 (BD Biosciences, Cat# 554285, RRID: AB_395344), FLAG (M2) (MilliporeSigma, Cat# P2983, RRID: AB_439685), RXRα (Abcam, Cat# ab125001, RRID: AB_10975632), pSer22 mouse RXRα (affinity-purified rabbit polyclonal antibody made to keyhole limpet hemocyanine conjugated to Cys-QVNSSSLNpSPTGR), and α-tubulin (MilliporeSigma, clone B-5-1-2, Cat# T5168, RRID: _477579). IRDye 680RD-conjugated goat anti-mouse IgG antibody (LI-COR Biosciences, Cat# 926-68070, RRID: AB_10956588) and IRDye 800CW-conjugated goat anti-rabbit IgG (LI-COR Biosciences, Cat# 926-32211, RRID: AB_621843) were used to detect immune complexes with the Odyssey infrared imaging system (LI-COR Biosciences).
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