P akt 193h12

Antibodies against full length APP (ab32136), BACE2 (ab8025), glucagon (ab10988), IDE (ab32216) and the insulin receptor (ab69508) were purchased from Abcam (Cambridge, UK). Antibodies against insulin (L6B10), p-GSK3β (D3A4), GSK3β (D5C5Z), p-AKT (193H12) and AKT (C67E7) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The monoclonal 4G8 antibody targeting the Aβ peptide used in these studies was purchased from Covance (Princeton, NJ, USA) and is now available from Biolegend (San Diego, CA, USA). Antibodies against the N-terminus of APP (MAB348), GLUT2 (07-1402), and GLUT4 (07-1404) were purchased from Millipore (Darmstadt, Germany). Antibodies against GLUT1 (sc-7903), GLUT3 (sc-7682), and ZNT8 (sc-98243) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).The antibody for amylin (250470) was purchased from Abbiotec (San Diego, CA, USA). The antibody against human APP (803001) was purchased from Biolegend (San Diego, CA, USA). The antibody against Aβ oligomers (11610) was purchased from Cayman Chemical (Ann Arbor, MI, USA). The antibody against neprilysin (MAB1126) was purchased from R&D Systems (Minneapolis, MN, USA).