Particle samples were dried in a 60 °C oven and secured to an aluminium sample holder with carbon tape and coated with 10 nm gold. Images were acquired using Zeiss Sigma-300 scanning electron microscope (SEM). For energy dispersive x-ray spectroscopy (EDX) analysis, samples were secured with silver conductive paint.
Sigma 300 scanning
Manufactured by Zeiss
The Sigma-300 is a scanning electron microscope (SEM) manufactured by Zeiss. It is designed to provide high-resolution imaging of samples at the nanoscale level. The Sigma-300 utilizes a field emission electron source to produce a focused electron beam, which is then scanned across the surface of the sample. The interactions between the electron beam and the sample generate signals that are detected and converted into an image, allowing for detailed analysis of the sample's surface topography and composition.
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Lab products found in correlation
2 protocols using sigma 300 scanning
1
SEM Imaging and EDX Analysis
2
Characterization of Anti-Pathogen Silver-Copper
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Microstructure observation was conducted for the developed anti-pathogen SS. Specimens were ground by abrasive papers up to 2000 grits and polished by 1 μm polishing liquid. The ZEISS Sigma 300 scanning electronic microscope (SEM) equipped with a backscattering electron (BSE) detector and energy dispersive X-ray spectroscopy (EDX) was used. Focus ion beam (FIB, FEI Helios 600i) was used to prepare transmission electron microscope (TEM) samples. Talos TEM equipped with a high-angle annular dark field (HAADF) detector and high-resolution EDX was used to identify the nano-sized precipitates.
The ISO standard 10993–12:2002 was used as the reference for the Ag/Cu ion release test. All testing samples were ground up to 2000 grit with an exposed surface area of about 1.96 cm2 and placed in a 24-well culture plate. Then, 1.5 mL of the as-prepared DMEM solution, which was used to prepare the SARS-CoV-2 virus-contained droplet, was dropped into each well containing samples. After that, the plate was also incubated at ambient conditions (at 22 ∼ 23 °C, 60 ∼ 70% relative humidity) for different time intervals. Finally, the released Cu and Ag ion concentration was measured by an inductively coupled plasma mass spectrometry (ICP-MS, iCAP RQ Quadrupole, ThermoFisher, USA). Three duplicate experiments were conducted for each material at the same time intervals.
The ISO standard 10993–12:2002 was used as the reference for the Ag/Cu ion release test. All testing samples were ground up to 2000 grit with an exposed surface area of about 1.96 cm2 and placed in a 24-well culture plate. Then, 1.5 mL of the as-prepared DMEM solution, which was used to prepare the SARS-CoV-2 virus-contained droplet, was dropped into each well containing samples. After that, the plate was also incubated at ambient conditions (at 22 ∼ 23 °C, 60 ∼ 70% relative humidity) for different time intervals. Finally, the released Cu and Ag ion concentration was measured by an inductively coupled plasma mass spectrometry (ICP-MS, iCAP RQ Quadrupole, ThermoFisher, USA). Three duplicate experiments were conducted for each material at the same time intervals.
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