All chemicals were obtained from suppliers (Sigma-Adrich, St. Louis, MO, USA, TCI, Ark). NMR spectra were recorded on a Bruker ECA-400 MHz (Bruker Co. Billerica, MA, USA) spectrometer with TMS as an internal standard. Chemical shifts were in ppm (δ), and coupling constants (J) were reported in Hertz (Hz). High-resolution electrospray ionization mass spectra (HR-ESI-MS) was performed on the Agilent G6230A TOF LC/MS (Agilent Technologies Co. Ltd., Santa Clara, CA, USA) using ESI ion source. Absorbances in vitro experiments were measured using a spectrophotometer (Thermo, Singapore Republic of Singapore). Column chromatography (CC) was performed using silica gel (Qingdao Marine Chemical Inc., Qingdao, China). Thin-layer chromatography (TLC) was performed with silica gel GF254 (Yantai Huayang New Material Technology Co., Ltd., Yantai, China). Data of in vitro experiments were analyzed and plotted using Graghpad Prism (version 8, GraphPad Software, San Diego, California, 2020).
Agilent g6230a tof lc ms
Manufactured by Agilent Technologies
Sourced in United States
The Agilent G6230A TOF LC/MS is a time-of-flight mass spectrometer designed for liquid chromatography-mass spectrometry (LC/MS) applications. It is capable of accurate mass measurements and high-resolution mass analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using agilent g6230a tof lc ms
1
Spectroscopic Characterization of Chemical Compounds
2
Comprehensive Analytical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HR-ESI-MS was recorded on an Agilent G6230A TOF LC/MS (Agilent Technologies Co., Ltd., CA, US). IR spectra were recorded on a Frontier FT-IR with KBr pellets. 1D and 2D NMR experiments were recorded on an AVANCE NEO 600 NMR and a Bruker ECA-400 NMR (Bruker BioSpin, MA, US). The chemical shifts were given in ppm (d), relative to TMS as an internal standard, and coupling constants are in Hz. A surveyor high performance liquid chromatography (Thermo, US) were used to record the UV spectra. Preparative HPLC separation was carried out on an LC-3000 liquid chromatography system equipped with a UV detector (Beijing Chuangxin Hengtong Technology Co., Ltd., Beijing, China). The column applied in this work was an Innoval C 18 column (21.2×250 nm, 5 μm, Tianjin Bona Aijieer Technology Co., Ltd., Tianjin, China). Column chromatography (CC) was carried out using silica gel (200-300 mesh, Marine Chemical Factory, Qingdao, China), Sephadex LH-20 (Pharmacia, Sweden), and macroporous resin AB-8 (Nan Kai College Chemical Inc., Tianjin, China), respectively. TLC was performed on GF254 plates pre-coated with silica gel 60 (5-20 μm, Yantai Huayang New Material Technology Co., Ltd., China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!