All 23 standard compounds used for the quantification analysis were purchased from Sigma-Aldrich (purity 99.0%, all chromatographic grade), including butanoic acid, acetic acid, thujone, methyl eugenol, p-cymene, α-pinene, diallyl disulphide, α-terpineol, β-caryophyllene, β-myrcene, 1,8-cineole, D-limonene, linalool, methional, nonanal, phenylethanal, ethyl caprylate, 4-methyl-5-thiazoleethanol, (E)-2-octenal, (E)-2-heptenal, humulene, acetophenone, and linalyl acetate. The internal standard (2,4,5-trimethylthiazole) and n-alkanes (C8–C30) used to calculate the retention index (RI) were of chromatographic grade and purchased from Sigma-Aldrich (St, Louis, MO, USA). Diethyl ether and n-pentane for dilution and preparation of sample were obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). High purity helium (99.999% purity) and nitrogen (99.99% purity) were purchased and produced by Beijing Tianlirenhe Trading Co., Ltd. (Beijing, China).
Thujone
Manufactured by Merck Group
Sourced in United States
Thujone is a chemical compound found in certain essential oils, such as those derived from the Thuja tree. It functions as a modulator of gamma-aminobutyric acid (GABA) receptors in the central nervous system. Thujone is commonly used in research applications involving the study of neurotransmitter systems and their effects on physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Linalool, by Merck Group (1 mentions)
Diethyl ether, by Thermo Fisher Scientific (1 mentions)
α terpineol, by Merck Group (1 mentions)
Nonanal, by Merck Group (1 mentions)
α pinene, by Merck Group (1 mentions)
Acetophenone, by Merck Group (1 mentions)
P cymene, by Merck Group (1 mentions)
β caryophyllene, by Merck Group (1 mentions)
1 8 cineole, by Merck Group (1 mentions)
Linalyl acetate, by Merck Group (1 mentions)
Methional, by Merck Group (1 mentions)
Butanoic acid, by Merck Group (1 mentions)
D limonene, by Merck Group (1 mentions)
Methyleugenol, by Merck Group (1 mentions)
Humulene, by Merck Group (1 mentions)
Diallyl disulphide, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Ethyl caprylate, by Merck Group (1 mentions)
β myrcene, by Merck Group (1 mentions)
4 methyl 5 thiazoleethanol, by Merck Group (1 mentions)
3 protocols using thujone
1
Quantification of Volatile Compounds
2
Comprehensive Analytical Standards Sourcing
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The methanol, isopropanol, dioxane, and dichloromethane were purchased from VWR International (Radnor, Pennsylvania-US). The acetonitrile and ethanol were purchased from Carlo Erba, (Cornaredo-Milano, Italy). The aluminum chloride was purchased from Alfa Aesar (Haverhill, MA, USA). The natrium carbonate and 2,4-Dinitrophenylhydrazine (2,4-DNPH) were purchased from Fluka-Fisher scientific (Rodano-Milano, Italy). The potassium acetate and methanesulfonic acid were purchased from Acros Organics-Fisher scientific (Rodano-Milano, Italy). The thujone, daidzin, genistin, daidzein, genistein standards, and ethyl decanoate were purchased from Sigma-Aldrich-Merck (St. Louis, MO, USA).
3
Neurological effects of plant alkaloids
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Male Wistar rats aged 8-10 weeks (bred at the Central Animal Facility of the UAE University) and at a body weight of 180-200 g were used. The animals were paired randomly and housed in pairs in an air-conditioned room with controlled temperature (24 ± 1 °C) and humidity (55 ± 15%) under a 12-h light/dark cycle (lights on at 07:00 h). The animals were given free access to food and water. Experiments were conducted between 9:00 and 15:00 h, and all procedures were performed in accordance with the guidelines of the European Communities Council Directive of 2010 (2010/63/EU) and were approved by the Institutional Animal Ethics Committee of the College of Medicine and Health Sciences/United Arab Emirates University (ERA-2015-3132) . Naïve rats were handled for a week before drug treatment began.
Thujone, nicotine, and methyllycaconitine (MLA) were purchased from Sigma (St.Louis, MO, USA). They were dissolved in isotonic saline and administered intraperitoneally (i.p.) at a volume of 1 ml/kg. All doses were expressed in terms of the free base. All experiments were conducted in a blinded fashion in which the operator was unaware of the specific treatment groups to which an animal belonged.
Thujone, nicotine, and methyllycaconitine (MLA) were purchased from Sigma (St.Louis, MO, USA). They were dissolved in isotonic saline and administered intraperitoneally (i.p.) at a volume of 1 ml/kg. All doses were expressed in terms of the free base. All experiments were conducted in a blinded fashion in which the operator was unaware of the specific treatment groups to which an animal belonged.
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