All MRI studies were performed using a 7.0-T small-animal MRI system (Agilent Technologies, USA) with a 160-mm bore magnet and a standard body coil. In CEST imaging experiments, we utilized an echo planar imaging (EPI) readout sequence with a frequency-selective continuous wave saturation pulse.
7.0 t small animal mri system
Manufactured by Agilent Technologies
Sourced in United States
The 7.0 T small-animal MRI system is a magnetic resonance imaging (MRI) device designed for small animal research applications. It provides a high-field strength of 7.0 Tesla, enabling high-resolution imaging of small subjects such as rodents and other small laboratory animals. The system is optimized for delivering consistent and reliable imaging data to support research studies.
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5 protocols using 7.0 t small animal mri system
1
Small-Animal 7.0-T MRI CEST Imaging
2
Quantifying Body Composition in Mice
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In the middle of the 13th week, proportions of the body fat and skeletal muscle in mice were analyzed by MRI. Briefly, mice were anesthetized by oxygen with 2% isoflurane at a flow rate of 1 L/min, and then scanned using a 7.0 T small animal MRI system (Agilent, Palo Alto, CA, USA). The images were captured with a T1-spin echo multislice sequence as follows: field of view =96.7 × 58.6 mm, no slices =34, slice thickness =0.6 mm (zero slice gap), repetition time =665.99 ms, echo time =13.44 ms, image matrix = 256 × 256, number of averages =6. The images were analyzed by Inveon Research Workplace 4.2 (Siemens, Germany), and percentages of the body fat and hindlimb skeletal muscle in mice were calculated.
3
7T MRI Volumetric Analysis of Tspan7-/- Rats
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The MRI examination was performed on an 7.0 T small-animal MRI system (Varian). During imaging, WT and Tspan7−/− rats (n = 6/group) were anesthetized with medical oxygen (1 Liter/min) containing 2% isoflurane. T2-weighted MRI images in the coronal plane were acquired with a fast spin echo (fsems) sequence with following parameters: FOV (field of view) = 35 × 35 mm; no slices = 20; slice thickness = 1 mm (zero slice gap); TR (repetition time) = 3,500 ms; TE (echo time) = 18.0 ms; image matrix = 256 × 256; and number of averages = 10. Volumes (region of the cortex, hippocampus, cerebellum, and cerebrum of WT and Tspan7−/− rats) were then drawn on the T2 images to determine the change of volumes (Vnmrj; Varian).
4
In Vivo MRI Analysis of Rodent Brains
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In vivo magnetic resonance imaging (MRI) was performed on a 7.0 T small animal MRI system (Varian, Palo Alto, CA, USA). For imaging, wild‐type and model rats (n = 4/group) were anesthetized with medical oxygen (1 L/min) containing 2% isoflurane. Fast spin‐echo (fsems) sequences were used to acquire coronal T2‐weighted MRI images with the following parameters: field of view = 35 × 35 mm; no. of slices = 20; slice thickness = 1 mm (zero slice gap); TR (repetition time) = 3000 ms; TE (echo time) = 18.0 ms; image matrix = 256 × 256; average = 10. Interesting regoins, such as corpus callosum, cortex, hippocampus, and ventricles, were plotted on T2 images to determine changes in signal intensity.
5
In Vivo 7.0-T Small-Animal MRI Protocol
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In vivo animal MRI was performed using a 7.0-T small-animal MRI system (Varian, Palo Alto, CA, USA). During imaging, the rats were anesthetized with medical oxygen (0.6 liter/min) containing 2% isoflurane. T2-weighted MRI images in coronal plane were acquired with a fast spin echo (fsems) sequence using the following parameters: FOV (field of view) = 35 by 35 mm; no slices = 20; slice thickness = 1 mm (zero slice gap); TR (repetition time) = 3500 ms; TE (echo time) = 18.0 ms; image matrix = 256 by 256; number of averages = 10.
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