Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin solution, and 0.25 % trypsin-EDTA were purchased from Invitrogen/Gibco (Grans Island, NY, USA). TNF-α, thiazolyl blue tetrazolium bromide (MTT), and 2',7'dichlorodihydrofluorescein diacetate (H 2 DCFDA) were purchased from Sigma Chemical (St.Louis, MO, USA). Tin protoporphyrin IX (SnPP) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Primary antibodies for CD54/ICAM-1, VCAM-1, HO-1, and glycerol-3-phosphate dehydrogenase (GAPDH) and goat anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Protease inhibitor cocktail tablets, CL-XPosre film, and the Super Signal West Pico Chemiluminescent substrate were obtained from Thermo Scientific (Waltham, MA, USA). Tin protoporphyrin IX (SnPP) was from Cayman Chemical (Ann Arbor, MI, USA).
Tin protoporphyrin 9
Manufactured by Cayman Chemical
Sourced in United States
Tin protoporphyrin IX is a laboratory reagent used in biochemical research. It is a metalloporphyrin compound that contains a tin ion at the center of the porphyrin ring structure. This compound is commonly used as a tool for studying heme-dependent enzymes and processes.
Automatically generated - may contain errors
Lab products found in correlation
Humid incubator, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
U0126, by Merck Group (1 mentions)
Indole 3 acetic acid, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Sartorius (1 mentions)
Anti nf κb p65, by Beyotime (1 mentions)
Ch223191, by MedChemExpress (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Nitric oxide assay kit, by Applygen (1 mentions)
Hieff qpcr sybr green master mix, by Yeasen (1 mentions)
Hifair 2 1st strand cdna synthesis supermix for qpcr, by Yeasen (1 mentions)
Anti ho 1, by ABclonal (1 mentions)
Protease inhibitor cocktail tablet, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg horseradish peroxidase hrp conjugated secondary antibodies, by Cell Signaling Technology (1 mentions)
Tnf α, by Merck Group (1 mentions)
3 protocols using tin protoporphyrin 9
1
Modulation of Inflammatory Responses
2
Dexamethasone-induced Apoptosis in Osteoblasts
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Mouse osteoblastic MC3T3-E1 cells were grown in Dulbecco's Modified Eagle's Medium (high glucose; Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% foetal bovine serum (Gibco, Thermo Fisher Scientific, Inc.), 100 U/ml penicillin (Sigma–Aldrich, St. Louis, MO, USA) and 100 μg/ml streptomycin (Sigma–Aldrich) in a humid incubator (Thermo Fisher Scientific, Inc.) with 5% CO2 at a temperature of 37°C. To induce apoptosis, MC3T3-E1 cells were treated with different concentrations of Dex (Sigma–Aldrich) for 24 h.
To induce HO-1 expression, CoPP (3 μM; Frontier Scientific, Logan, USA) was added to the culture 12 h before Dex treatment. In some experiments, U0126 (an inhibitor of ERK activation, 10 μM; Sigma–Aldrich) or tin protoporphyrin IX (an inhibitor of HO-1, SnPP, 20 μM; Cayman Chemical, Ann Arbor, MI, USA) was added to the culture 1 h before CoPP treatment.
To induce HO-1 expression, CoPP (3 μM; Frontier Scientific, Logan, USA) was added to the culture 12 h before Dex treatment. In some experiments, U0126 (an inhibitor of ERK activation, 10 μM; Sigma–Aldrich) or tin protoporphyrin IX (an inhibitor of HO-1, SnPP, 20 μM; Cayman Chemical, Ann Arbor, MI, USA) was added to the culture 1 h before CoPP treatment.
3
Indole-3-acetic acid and LPS Inflammation
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Indole-3-acetic acid and LPS (lipopolysaccharides from Escherichia coli O55:B5) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Tin protoporphyrin IX (SnPP) was obtained from Cayman Chemical (Ann Arbor, MI, USA). CH-223191 was purchased from MedChem Express (Monmouth Junction, NJ, USA). Dulbecco’s modified Eagle’s medium (DMEM) was from Biological Industries (Kibbutz Beit Haemek, Israel). Fetal bovine serum (FBS) was purchased from Hyclone (South Logan, UT, USA). Cell counting kit (CCK-8), TRIzol RNA extraction reagent, nitric oxide assay kit, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), and Hoechst 33,342 were obtained from Applygen Technologies Inc. (Beijing, China). Hieff® qPCR SYBR® Green Master Mix and Hifair® II 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus) were procured from Yeasen biotech Co. (Shanghai, China). Antibody against β-actin was from Santa Cruz Bio. (Santa Cruz, CA, USA). Anti-HO-1 was purchased from ABclonal Inc. (College Park, MD, USA). Anti-NF-κB p65 was from Beyotime (Shanghai, China). ECL Western blotting detection kit, horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG), and anti-mouse IgG were purchased from Huaxingbio. (Beijing, China). ELISA kits for IL-1β, IL-6, TNF-α, and MCP-1 were bought from ExCell Biotech (Taicang, Jiangsu, China).
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