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T1 inverted fluorescence microscope

Manufactured by Nikon
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The Nikon T1 is an inverted fluorescence microscope designed for laboratory use. It features a motorized stage and advanced optics for high-quality imaging of fluorescently labeled samples. The T1 is capable of providing clear, detailed views of cellular structures and processes.

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Lab products found in correlation

Ds qi2 cmos camera, by Nikon (3 mentions) Lab tek eight well chambers, by Thermo Fisher Scientific (2 mentions) Nis elements ar software, by Nikon (1 mentions) Elements ar software, by Nikon (1 mentions) Deconvolution software, by Scientific Volume Imaging (1 mentions) Element ar software, by Nikon (1 mentions)

Close spelling variants of this product

Fluorescence microscope (1854 citations) Inverted microscope (910 citations) Inverted fluorescence microscope (437 citations)

4 protocols using t1 inverted fluorescence microscope

1

Live-cell Imaging of Protein Translocation

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Live transfected cells cultured on poly(d‐lysine)‐coated glass coverslips (Hecht Assistant) were treated with either 100 nM rapamycin for rapid induction of protein dimerization and translocation or microtubule‐targeting agents (3.3 μM nocodazole or 500 μM colchicine) during imaging. Live‐cell imaging was conducted on a Nikon T1 inverted fluorescence microscope (Nikon) with a 60× oil objective (Nikon), a Prime camera (Photometrics), and 37°C, 5% CO2 heat stage (Live Cell Instrument). Rapid recruitment of POIs was imaged at 5‐ or 10‐s intervals, whereas the process of microtubule disruption was imaged at 1‐min intervals. Images were obtained using Nikon NIS‐Elements AR software and processed with Huygens Deconvolution Software (Scientific Volume Imaging). Image analysis was mainly performed with Nikon NIS‐Elements AR software.
Liu G.Y., Chen S., Lee G., Shaiv K., Chen P., Cheng H., Hong S., Yang W., Huang S., Chang Y., Wang H., Kao C., Sun P., Chao M., Lee Y., Tang M, & Lin Y. (2022). Precise control of microtubule disassembly in living cells. The EMBO Journal, 41(15), e110472.
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2

Live-cell imaging of cytoskeletal dynamics

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Neon-IFT88 or Neon-Kif3B-stable NIH3T3 cells were transfected with the indicated constructs and then seeded onto poly(d-lysine)-coated borosilicate glass Lab-Tek eight-well chambers (Thermo Scientific). The cells were imaged every 200 ms for 30 s on a Nikon T1 inverted fluorescence microscope (Nikon) with a ×60 oil objective (Nikon), DS-Qi2 CMOS camera (Nikon), and 37 °C, 5% CO2 heat stage (Live Cell Instrument). Time-lapse images were processed by Huygens deconvolution (Scientific Volume Imaging), and kymographs were produced with ImageJ and the plug-in KymographClear70 (link).
Hong S.R., Wang C.L., Huang Y.S., Chang Y.C., Chang Y.C., Pusapati G.V., Lin C.Y., Hsu N., Cheng H.C., Chiang Y.C., Huang W.E., Shaner N.C., Rohatgi R., Inoue T, & Lin Y.C. (2018). Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling. Nature Communications, 9, 1732.
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3

Live-cell Imaging with Nikon Microscope

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Live‐cell imaging was carried out with a Nikon T1 inverted fluorescence microscope with a 60× or 100× oil objective (Nikon), DS‐Qi2 CMOS camera (Nikon), and 37°C, 5% CO2 heated stage (Live Cell Instrument). Images with multiple z‐stacks were processed with Huygens Deconvolution software (Scientific Volume Imaging). Image analysis and the maximum intensity projections of images were generated with Nikon Elements AR software.
Cheng H., Kao Y., Chen T., Sharma L., Yang W., Chuang Y., Huang S., Lin H., Huang Y., Kao C., Yang L., Bearon R., Cheng H., Hsia K, & Lin Y. (2022). Actin filaments form a size‐dependent diffusion barrier around centrosomes. EMBO Reports, 24(1), e54935.
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4

Live-cell Fluorescence Microscopy Protocol

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Cells were plated on poly(d-lysine)-coated borosilicate glass Lab-Tek eight-well chambers (Thermo Scientific). Live-cell imaging was performed using a Nikon T1 inverted fluorescence microscope (Nikon) with a ×60 oil objective (Nikon), DS-Qi2 CMOS camera (Nikon), and 37 °C, 5% CO2 heat stage (Live Cell Instrument). Imaging was acquired using Nikon element AR software. Images with multiple z-stacks were processed with Huygens deconvolution (Scientific Volume Imaging), and the maximum intensity projections of images were produced by Nikon element AR software (Nikon). The image analysis was mainly conducted by Nikon element AR software (Nikon).
Hong S.R., Wang C.L., Huang Y.S., Chang Y.C., Chang Y.C., Pusapati G.V., Lin C.Y., Hsu N., Cheng H.C., Chiang Y.C., Huang W.E., Shaner N.C., Rohatgi R., Inoue T, & Lin Y.C. (2018). Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling. Nature Communications, 9, 1732.
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