Hemoglobin

Manufactured by Tecan

Sourced in Switzerland

Hemoglobin is a protein found in red blood cells that is responsible for transporting oxygen throughout the body. It is a key component in the analysis and measurement of various blood parameters in a laboratory setting.

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Lab products found in correlation

Magellan v7, by Tecan (2 mentions) Human hemoglobin, by Merck Group (2 mentions) Sodium dodecyl sulfate (sds), by Merck Group (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Infinite m nano, by Tecan (1 mentions) Liquichip 200 instrument, by Qiagen (1 mentions) Infinite m nano plate reader, by Tecan (1 mentions)

2 protocols using hemoglobin

Quantifying Alveolar Hemorrhage from BALF

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BALF total protein was measured with a Pierce BCA Protein Assay Kit (Thermo Scientific). Alveolar hemorrhage was assessed as described elsewhere (32 (link)), but with the following modifications. A 6X 2-fold serial dilution (8000–250 μg/mL) of human hemoglobin (Sigma, St. Louis, MO) was prepared, and 50 μL of each standard and BALF sample were added to duplicate wells of a 96-well plate. A volume of 100 μL of 6% sodium dodecyl sulfate (Sigma, St. Louis, MO) was added to all wells and resuspended several times. The absorbance at 560 nm was measured with a Tecan Infinite M Nano plate reader (Tecan, Männedorf, Switzerland), and alveolar hemorrhage (i.e., total amount of hemoglobin recovered in BALF) was determined from the standard curve generated by Magellan V 7.2 (Tecan, Männedorf, Switzerland) software.

Sharma A., Noon J.B., Kontodimas K., Garo L.P., Platten J., Quinton L.J., Urban JF J.r., Reinhardt C, & Bosmann M. (2022). IL-27 enhances γδ T cell-mediated innate resistance to primary hookworm infection in the lungs. Journal of immunology (Baltimore, Md. : 1950), 208(8), 2008-2018.

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Quantifying Alveolar Hemorrhage and Cytokines

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BALF total protein was measured with a Pierce BCA Protein Assay Kit (Thermo Scientific).
Alveolar hemorrhage was assessed as described elsewhere (30) , but with the following modifications. A 6X 2-fold serial dilution (8000-250 µg/mL) of human hemoglobin (Sigma, St. Louis, MO) was prepared, and 50 µL of each standard and BAL sample was added to duplicate wells of a 96-well plate. A volume of 100 µL of 6% sodium dodecyl sulfate (Sigma) was added to all wells and resuspended several times. The absorbance at 560 nm was measured with a Tecan Infinite M Nano plate reader (Tecan, Männedorf, Switzerland), and alveolar hemorrhage (i.e., total amount of hemoglobin recovered in BAL) was determined from the standard curve generated by Magellan V 7.2 (Tecan) software. (CCL-7), MIP-1α (CCL-3), MIP-1β (CCL-4), MIP-2 (CXCL-2), RANTES (CCL-5), Eotaxin (CCL-11), GM-CSF, IFN gamma, and TNF alpha (31) . All samples from bead-based assays were performed using a LiquiChip-200 instrument (Qiagen, Hilden, Germany) using Bio-Plex Manager v6.1 software for quantification.

Noon J., Sharma A., Platten J., Quinton L.J., Reinhardt C., & Bosmann M. (2020). IL-27 enhances the lymphocyte mediated innate resistance to primary hookworm infection in the lungs.

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