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Two step luminescence assay

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The Two-step luminescence assay is a laboratory instrument designed to measure luminescent signals in biological samples. It performs a two-step process to quantify the intensity of luminescence, which can be used to analyze various biomolecular interactions and reactions.

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Lab products found in correlation

U ch1, by American Type Culture Collection (2 mentions) U ch2, by American Type Culture Collection (2 mentions)

2 protocols using two step luminescence assay

1

Chordoma Cell Line Validation Protocol

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UM-Chor1 (clival) and JHC7 (sacral) were kindly provided by the Chordoma Foundation (Durham, NC) on approximately 4/2/2015 with STR validation. U-CH1 (sacral), U-CH2 (sacral), UM-Chor5 (clival), and MUG-CC1 (clival) were obtained from American Type Culture Collection on approximately 7/20/2020 with STR validation. All cell lines were passaged twice per week, used at a low (<30) passage number, and serially verified to be free of mycoplasma infection using a two-step luminescence assay (Lonza Bioscience).
Hoke A.T., Padget M.R., Fabian K.P., Nandal A., Gallia G.L., Bilusic M., Soon-Shiong P., Hodge J.W, & London NR J.r. (2021). Combinatorial Natural Killer Cell–based Immunotherapy Approaches Selectively Target Chordoma Cancer Stem Cells. Cancer Research Communications, 1(3), 127-139.
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2

Chordoma Cell Line Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
UM-Chor1 (clival) and JHC7 (sacral) were kindly provided by the Chordoma Foundation on approximately April 2, 2015 with short tandem repeat (STR) validation. U-CH1 (sacral), U-CH2 (sacral), UM-Chor5 (clival), and MUG-CC1 (clival) were obtained from ATCC on approximately July 20, 2020 with STR validation. All cell lines were passaged twice per week, used at a low (<30) passage number, and serially verified to be free of Mycoplasma infection using a two-step luminescence assay (Lonza Bioscience).
Hoke A.T., Padget M.R., Fabian K.P., Nandal A., Gallia G.L., Bilusic M., Soon-Shiong P., Hodge J.W, & London NR J.r. (2021). Combinatorial Natural Killer Cell–based Immunotherapy Approaches Selectively Target Chordoma Cancer Stem Cells. Cancer Research Communications, 1(3), 127-139.
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