The protocol of real-time reverse transcription-PCR (RT-PCR) analysis was slightly modified from that performed by Porro and colleagues (27 (link)). Total RNA of blood samples were isolated with the Trizol reagent, respectively (Invitrogen, Carlsbad, CA), and reverse transcribed with β-actin and telomere-specific oligonucleotides using HiScript SuperMix for qPCR kit (Vazyme Biotech Co., Ltd, Nanjing, China). cDNA was amplified using the SYBR green master mix (Vazyme Biotech Co., Ltd, Nanjing, China) with the primers listed in Supplementary Table 4 and analyzed using the Rotor gene Q 2000 (QIAGEN, Hilden, Germany) with the following thermal cycling profile: Stage 1: 2 minutes at 95°C; Stage 2: 40 cycles of 15 seconds at 98°C, 20 seconds at 60°C, 1 minute at 74°C with signal acquisition.
Hiscript supermix for qpcr kit
Manufactured by Vazyme
Sourced in China
HiScript SuperMix for qPCR is a ready-to-use solution containing all the necessary components for quantitative real-time PCR (qPCR) reactions, including DNA polymerase, dNTPs, and reaction buffer. It is designed to provide efficient and reliable amplification for quantitative gene expression analysis.
Automatically generated - may contain errors
2 protocols using hiscript supermix for qpcr kit
1
Real-Time RT-PCR Analysis of Blood RNA
2
Quantifying Gene Expression via RT-qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cDNA synthesis was performed using HiScript SuperMix for qPCR kit (Vazyme, China), RT‐qPCR was performed using a Roche 480 real‐time PCR detection system (Roche, Switzerland). Results were normalized to GAPDH and were analyzed using the 2−△△Ct method. The primer sequences are listed in Table S1 , Supporting Information.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!