V10 flow cytometry analysis software

Cells infected with GFP-producing rBEVs were analyzed using a FACSCalibur™ flow cytometer (BD Biosciences, San Jose, CA, USA) equipped with an argon-ion laser with an excitation frequency of 488 nm. Samples were run at the low flow setting (12 $\mathsf{\mu }$ L/min) and 10,000 events were collected. Analysis of flow cytometry data was performed using FlowJo^® V10 flow cytometry analysis software (FlowJo LLC, Ashland, OR, USA). Results are reported as the mean of at least 3 independent replicates.

Fluorescent cells were acquired using a BD Accuri™ C6 Plus flow cytometer (BD Biosciences, San Jose, CA, USA) equipped with 488 nm and 640 nm lasers. Samples were run at the low flow setting and 10,000 events were collected and analyzed using FlowJo^® V10 flow cytometry analysis software (FlowJo LLC, Ashland, OR, USA).

Samples were analyzed using a FACSCalibur™ flow cytometer (BD Biosciences, San Jose, CA, USA) equipped with a 15 mW air-cooled argon-ion laser with an excitation frequency of 488 nm. Samples were run at the low flow setting (12 µL/min), and 10,000 events were collected. The analysis of flow cytometry data was performed using FlowJo^® V10 flow cytometry analysis software (FlowJo LLC, Ashland, OR, USA). Briefly, after applying gates to remove debris and intrinsic cellular fluorescence from the analysis, median fluorescence intensity in FL1 was calculated. Where appropriate, tests for statistical significance of differences in fluorescence intensity were performed using Student’s t-test, with a significance level value of 5%.