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Biotinylated igg1

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Biotinylated-IgG1 is a biotin-labeled immunoglobulin G1 (IgG1) molecule. It is a laboratory reagent used for various applications in biotechnology and research, where the biotin label can be utilized for detection or purification purposes.

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Lab products found in correlation

Biotinylated anti mouse igm, by Jackson ImmunoResearch (3 mentions) Alkaline phosphatase conjugated anti mouse igg, by Thermo Fisher Scientific (3 mentions) Biotinylated igg2b, by Southern Biotech (3 mentions) Biotinylated anti kappa ab, by Thermo Fisher Scientific (2 mentions) Anti igm or anti igg capture antibodies, by Thermo Fisher Scientific (2 mentions) Ap igg2c abs, by Southern Biotech (2 mentions) Streptavidin sa alkaline phosphatase, by Vector Laboratories (2 mentions) Elisa plate, by Thermo Fisher Scientific (1 mentions) Salmon sperm dsdna, by Thermo Fisher Scientific (1 mentions) Streptavidin alkaline phosphatase, by Vector Laboratories (1 mentions) Biotinylated anti κ abs, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Biotinylated goat-anti-mouse IgG1 (4 citations) Biotinylated anti-mouse IgG1 (2 citations)

4 protocols using biotinylated igg1

1

Quantifying Mouse Autoantibody Isotypes by ELISA

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ELISA plates were coated with anti-IgM or anti-IgG capture antibodies (from Invitrogen, Grand Island, NY) and detected using biotinylated anti-mouse IgM (Jackson Immunoresearch, West Grove, PA) or alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Total IgG autoAb titers were measured in ELISA plates coated with dsDNA, histone, nucleosome, Sm/RNP or cardiolipin and detected with biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY). IgG subtype-specific autoAb titers were detected by biotinylated-IgG1, biotinylated IgG2b, and AP-IgG2c Abs (Southern Biotech, Birmingham, AL). Biotinylated antibodies were detected by streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed by the PNPP (p-Nitrophenyl Phosphate, Disodium Salt) (Thermo Fisher Scientific, Rockford, IL) substrates for alkaline phosphatase.
Wong E.B., Soni C., Chan A.Y., Domeier P.P., S.h.w.e.t.a.n.k., Abraham T., Limaye N., Khan T.N., Elias M.J., Chodisetti S.B., Wakeland E.K, & Rahman Z.S. (2015). B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance. Journal of immunology (Baltimore, Md. : 1950), 194(9), 4130-4143.
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2

Antigen-Specific IgG Subclass Analysis

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To identify the antigen-specific antibody per IgG subclasses, biotinylated IgG1 (Southern Biotech Cat# 9052–08) was added at 5ug/ml and biotinylated IgG2, IgG3, or IgG4, specific detection reagents (Southern Biotech Cat#s 9052–08, 9060–08, 9210–08, 9200–08) were added at 20 ug/ml in 2% Prionex and a customized Luminex subclassing assay was used as described previously [18 (link)].
Ravindran R., McReynolds C., Yang J., Hammock B.D., Ikram A., Ali A., Bashir A., Zohra T., Chang W.L., Hartigan-O’Connor D.J., Rashidi H.H, & Khan I.H. (2021). Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses. PLoS ONE, 16(7), e0254367.
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3

Quantification of serum IgM/IgG and autoantibodies

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Total serum IgM/IgG or IgG from in vitro B cell culture supernatants was measured using standard ELISA protocols. Serum Ab titers were quantitated as described (29 ). Briefly, ELISA plates were coated with anti-IgM or anti-IgG capture antibodies (from Invitrogen, Grand Island, NY) and detected using biotinylated anti-mouse IgM (Jackson Immunoresearch, West Grove, PA) or alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Total IgG autoAb titers were measured in ELISA plates coated with dsDNA, histone, nucleosome, Sm/RNP or cardiolipin and detected with biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY). IgG subtype-specific autoAb titers were detected by biotinylated-IgG1, biotinylated IgG2b, and AP-IgG2c Abs (Southern Biotech, Birmingham, AL). Biotinylated antibodies were detected by streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed by the PNPP (p-Nitrophenyl Phosphate, Disodium Salt) (Thermo Fisher Scientific, Rockford, IL) substrates for alkaline phosphatase.
Soni C., Wong E.B., Domeier P.P., Khan T.N., Satoh T., Akira S, & Rahman Z.S. (2014). B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. Journal of immunology (Baltimore, Md. : 1950), 193(9), 4400-4414.
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4

Sensitive ELISA for Autoantibody Detection

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For IgG or IgM detection, ELISA plates (Thermo Fisher Scientific) were coated with anti-IgM or anti-IgG (both from Invitrogen) capture Abs and detected using biotinylated anti–mouse IgM (Jackson ImmunoResearch Laboratories, Inc.) or alkaline phosphatase–conjugated anti–mouse IgG (Molecular Probes). Total IgG auto-Ab titers were measured in ELISA plates coated with salmon sperm dsDNA (Invitrogen), histone (Sigma-Aldrich), or nucleosome (histone plated on a layer of dsDNA coating) and detected with biotinylated anti-κ Abs (Invitrogen). IgG subtype–specific auto-Ab titers were detected by biotinylated IgG1, biotinylated IgG2b, and alkaline phosphatase IgG2c Abs (SouthernBiotech). Biotinylated Abs were detected by streptavidin–alkaline phosphatase (Vector Laboratories). The plates were developed by the PNPP (p-nitrophenyl phosphate, disodium salt; Thermo Fisher Scientific) substrates for alkaline phosphatase and read at 405 nm.
Domeier P.P., Chodisetti S.B., Soni C., Schell S.L., Elias M.J., Wong E.B., Cooper T.K., Kitamura D, & Rahman Z.S. (2016). IFN-γ receptor and STAT1 signaling in B cells are central to spontaneous germinal center formation and autoimmunity. The Journal of Experimental Medicine, 213(5), 715-732.
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