Serum free cho s sfm 2

The mammalian expression vector, pcDNA3, CHO-K1 suspension (CHO-S) cells,
FreeStyle MAX reagent, FreeStyle CHO expression medium, pCMV-ARMS1-PK2
expression vector, antibiotics, and AssayComplete medium were purchased from
Invitrogen Corporation (San Diego, CA, USA). The following reagents and
materials were also used: restriction enzymes and a DNA ligation kit (Takara,
Tokyo, Japan); CHO cells (Japanese Cancer Research Resources Bank, Tokyo,
Japan); and Ham's F-12 medium, Opti-MEM I, serum-free CHO-S-SFM II,
Geneticine, and Lipofectamine 2000 (Gibco BRL, Grand Island, NY, USA). Fetal
bovine serum (FBS) was obtained from Hyclone Laboratories (Logan, UT, USA).
Pro-Prep^TM protein-extraction solution was obtained from Intron
Biotechnology (Seoul, Korea). Lumi-Light western blot kit was purchased from
Roche (Basel, Switzerland). PathHunter CHO-K1 β-arrestin Parental cell
line was obtained from DiscoveRx (San Diego, CA, USA). Disposable spinner flasks
and grass flasks were obtained from Corning Incorporated (NY, USA). The cAMP
Dynamic 2 immunoassay kit was purchased from Cisbio Bioassay (Codolet, France).
All other reagents used were obtained from Sigma-Aldrich Corp (St. Louis, MO,
USA). The oligonucleotides used in this study were synthesized by Genotech
(Daejon, Korea).

The pcDNA3 mammalian expressing vector, Chinese hamster ovary suspension (CHO-S)
cells, FreeStyle MAX reagent, and FreeStyle CHO expression medium, were
purchased from Invitrogen (San Diego, CA, USA). CHO-K1 cells were obtained from
the Korean Cell Bank (KCB) (Seoul, Korea). The polymerase chain reaction (PCR)
reagents, restriction enzymes, and DNA ligation kits were obtained from Takara
(Shiga, Japan). Ham’s F-12 medium, Opti-MEM I, serum-free CHO-S-SFM II,
and Lipofectamine 2000 were purchased from Gibco BRL (Grand Island, NY, USA).
Fetal bovine serum (FBS) was obtained from Hyclone Laboratories (Logan, UT,
USA). Disposable spinner flasks and glass flasks were obtained from Corning
(Corning, NY, USA). The cyclic adenosine monophosphate (cAMP) Dynamic 2
immunoassay kit was purchased from Cisbio Bioassay (Codolet, France). PSMG
enzyme-linked immunosorbent assay (ELISA) kit was purchased from DRG
International (Mountainside, NJ, USA). The QIAprep-Spin plasmid kit was
purchased from Qiagen (Hilden, Germany). The oligonucleotides were synthesized
by Genotech (Daejeon, Korea). All other reagents were obtained from
Sigma-Aldrich (St. Louis, MO, USA).