Table s2

Manufactured by Integrated DNA Technologies

Sourced in United States

The Table S2 is a laboratory equipment product offered by Integrated DNA Technologies. It serves as a centrifuge table, providing a stable and secure platform for centrifuge equipment used in various laboratory workflows. The core function of the Table S2 is to support and hold centrifuge devices, facilitating efficient and controlled sample processing.

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Lab products found in correlation

Evagreen supermix, by Bio-Rad (1 mentions) Superscript 3 supermix, by Thermo Fisher Scientific (1 mentions) γ 32p atp 6000 ci mmol, by PerkinElmer (1 mentions) T4 polynucleotide kinase, by New England Biolabs (1 mentions)

2 protocols using table s2

Quantitative Microbial Gene Expression Analysis

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Droplet digital PCR (ddPCR) of hydrazine synthase (hzsB), ammonia monoxygenase (amoA), nosZ clade I genes, and transcripts used the QX200 platform with 20 µl reactions, 10 µl 2× EvaGreen Supermix (Biorad, Hercules, CA, US), and 1 µl DNA or cDNA. RNA (24 pg–1.1 µg) was converted to cDNA using Superscript III Supermix (Invitrogen). DPEC-treated water was used for negative controls and gBlock dsDNA fragments were used as positive controls (Table S2; Integrated DNA Technologies, Coralville, IA, USA). Primers and PCR conditions are described in Table S2. Data were analyzed using the QuantaSoft software package v1.0 (Bio-Rad). Positive droplet thresholds were set based on negative and positive droplet fluorescence amplitudes using the positive control as reference.

Mosley O.E., Gios E., Close M., Weaver L., Daughney C, & Handley K.M. (2022). Nitrogen cycling and microbial cooperation in the terrestrial subsurface. The ISME Journal, 16(11), 2561-2573.

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Preparation of Radiolabeled RNA Substrates

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RNAs for dicing assays (Table S2, Integrated DNA Technologies) were 5′ ³²P radiolabeled using [γ−³²P]ATP (6000 Ci/mmol) (PerkinElmer, Waltham, MA) and T4 polynucleotide kinase (NEB, Ipswich, MA), and then gel-purified. Substrates containing two ³²P-radiolabeled phosphates were prepared by DNA-splinted ligation (Moore and Sharp, 1993 (link); Moore and Query, 2000 (link)). Synthetic hairpins for substrate competition assays (Table S2) were ordered from Integrated DNA Technologies as HPLC-purified RNAs.

Jouravleva K., Golovenko D., Demo G., Dutcher R.C., Tanaka Hall T.M., Zamore P.D, & Korostelev A.A. (2022). Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB. Molecular cell, 82(21), 4049-4063.e6.

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