Ml385 sml1833

Manufactured by Merck Group

Sourced in United States

The ML385 (SML1833) is a compact, versatile laboratory instrument designed for a range of analytical applications. It features a high-resolution detector and automated calibration capabilities to ensure precise and reliable measurements. The core function of this product is to provide accurate and consistent data for researchers and scientists working in various fields.

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Lab products found in correlation

Ly294002 l9908, by Merck Group (3 mentions) Cd4 t cell isolation kit 2, by Miltenyi Biotec (3 mentions) Rna extraction kit, by Qiagen (3 mentions) Sa β gal staining kit, by Beyotime (3 mentions) P gsk3β, by Cell Signaling Technology (1 mentions) Histon h3, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) H1563, by American Type Culture Collection (1 mentions) H1299, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Rpmi 1640, by Lonza (1 mentions)

Close spelling variants of this product

ML385 (32 citations) SML1833 (2 citations)

4 protocols using ml385 sml1833

Senescence Regulation by Nrf2 and Akt

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RNA extraction kit was from Qiagen (Qiagen Inc., CA, USA). DAPI (#4083) and antibodies against Nrf2 (#12721), Akt (#9272), HO-1 (#43966), CAT (#14097), GSK-3β (#12456), NQO1 (#62262), P16 (#80772), P21 (#2947), p-Akt (#4060), p-GSK-3β (#5558), Fyn (#4023), Histon H3 (#4499), and β-actin (#4970) were obtained from Cell Signalling Technology Corporation (MA, USA). CD4⁺ T Cell Isolation Kit II was obtained from Miltenyi (Bergisch Gladbach, Germany). SA-β-Gal staining kit was obtained from Beyotime (Shanghai, China). LY294002 (L9908) and ML385 (SML1833) were obtained from Sigma-Aldrich (St. Louis., MO, USA).

Xiong Y., Wang Y., Zhang J., Zhao N., Zhang H., Zhang A., Zhao D., Yu Z., Yin Y., Song L., Xiong Y, & Luan X. (2020). hPMSCs protects against d-galactose-induced oxidative damage of CD4+ T cells through activating Akt-mediated Nrf2 antioxidant signaling. Stem Cell Research & Therapy, 11, 468.

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Cell Line Characterization and Culture

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A549 (ATCC: CCL-185), H1299 (ATCC: CRL-5803), and H1563 (ATCC: CRL-5875) NSCLC cell lines were purchased directly from ATCC. All cell lines were initially expanded and low passage numbers aliquots were frozen back to ensure experiments were conducted in cell lines with similar passage numbers. Cell lines were screened for mycoplasma at regular intervals, including when cell lines were frozen back. All cells were grown in RPMI 1640 (Lonza, Basel, Switzerland: 12-167F) with 10% Fetal Bovine Serum (Sigma-Aldrich, St. Louis MO, USA: F0926), Penicillin/Streptomycin (Gibco, Waltham, MA, USA: 15140-122), and 2 mM Glutamax (Gibco: 35050-061) and were maintained in a 37 °C humidified incubator with 5% CO₂. Purified artesunate (HY-N0193) was purchased from MedChem Express (Monmouth Junction, NJ, USA) and ML385 (SML1833) was purchased from Sigma-Aldrich.

Hill K.S., McDowell A., McCorkle J.R., Schuler E., Ellingson S.R., Plattner R, & Kolesar J.M. (2021). KEAP1 Is Required for Artesunate Anticancer Activity in Non-Small-Cell Lung Cancer. Cancers, 13(8), 1885.

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Cellular Senescence Pathway Analysis

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RNA extraction kit was from Qiagen (Qiagen Inc, CA, USA). DAPI(#4083) and antibodies against Nrf2 (#12721), Akt (#9272), HO-1 (#43966), CAT (#14097), GSK-3β (#12456), NQO1 (#62262), P16 (#80772), P21 (#2947), p-Akt (#4060), p-GSK-3β (#5558), Fyn (#4023), Histon H3 (#4499) and β-actin (#4970) were obtained from Cell signing technology corporation (Massachusetts, USA). CD4 + T Cell Isolation Kit II were obtained from Miltenyi (Bergisch Gladbach, Germany). SA-β-Gal staining kit were obtained from Beyotime (Shanghai, China). LY294002 (L9908) and ML385 (SML1833) were obtained from Sigma-Aldrich (St. Louis., MO, USA).

Xiong Y., Wang Y., Zhang J., Zhao N., Zhang A., Zhao D., Yu Z., Yin Y., Song L., Xiong Y., & Luan X. (2020). hPMSCs protects against D-galactose-induced oxidative damage of CD4+ T cells through activating Akt-mediated Nrf2 antioxidant signaling.

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Cellular Senescence Pathway Analysis

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Xiong Y., Wang Y., Zhang J., Zhao N., Zhang H., Zhang A., Zhao D., Yu Z., Yin Y., Song L., Xiong Y., & Luan X. (2020). hPMSCs protects against D-galactose-induced oxidative damage of CD4+ T cells through activating Akt-mediated Nrf2 antioxidant signaling .

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