Plasma insulin levels were measured using the Ultra-Sensitive Mouse Insulin enzyme-linked immunosorbent assay (ELISA) kit (Crystal Chem, Elk Grove Village, IL, USA). Murine plasma adiponectin was determined by ELISA according to the instructions of the manufacturer (Thermo Fisher Scientific, Vienna, Austria). Plasma tumour necrosis factor (TNF)-α levels were quantified using Mouse TNF-α Platinum ELISA (Thermo Fisher Scientific, Vienna, Austria).
Mouse tnf α platinum elisa
Manufactured by Thermo Fisher Scientific
Sourced in Austria
The Mouse TNF-α Platinum ELISA is a quantitative in vitro enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse tumor necrosis factor-alpha (TNF-α) in cell culture supernatants, serum, and plasma samples.
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Lab products found in correlation
3 protocols using mouse tnf α platinum elisa
1
Plasma Biomarkers Quantification
2
Plasma Metabolic Biomarkers in Mice
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Plasma insulin levels in C57BL/6J LDLr−/− mice were measured using the Ultra Sensitive Mouse Insulin enzyme-linked immunosorbent assay (ELISA) Kit (Crystal Chem, Elk Grove Village, IL, USA). Murine plasma adiponectin was determined by ELISA according to the instructions of the manufacturer (Thermo Fisher Scientific, Vienna, Austria). Plasma tumor necrosis factor (TNF)-α levels were quantified using the Mouse TNF-α Platinum ELISA (Thermo Fisher Scientific, Vienna, Austria).
3
Cytokine and Albumin Measurement in Biological Fluids
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Cytokines in thawed plasma (dilution, 1:10) and lung lavage fluid (dilution, 1:2) were measured by using sandwich ELISA kits according to the manufacturer's instructions. Albumin was measured in thawed plasma (dilution, 1:250,000) and lung lavage fluid (dilution, 1:1000). The assays used and their lower limit of detection (LLD) were: Mouse IL-6 DuoSet ELISA kit (LLD, 15.6 pg/mL; R and D Systems, Minneapolis, MN); Mouse Intracellular Adhesion Molecule-1 (ICAM-1) DuoSet ELISA kit (LLD 125 pg/mL; R and D Systems); Mouse Receptor for Advanced Glycation End-products (RAGE) DuoSet ELISA kit (LLD, 125 pg/mL; R and D Systems); Mouse IL-1β Platinum ELISA (LLD, 7.8 pg/mL; Thermo Fisher); Mouse TNF-α Platinum ELISA (LLD, 31.3 pg/mL; Thermo Fisher); and Mouse Albumin ELISA kit (LLD, 1.23 ng/mL; Bethyl Laboratories, Montgomery, TX).Absorbance was read on a spectrophotometer (Biotek, Winooski, VT) at a measuring wavelength of 450 nm for all ELISAs except IL-1β and TNF-α (reference wavelength 630 nm).The data were analyzed by using KC4 software (Biotek).
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