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Fitcconjugated dextran 40 kda

Manufactured by Merck Group
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FITC-conjugated dextran (40 kDa) is a fluorescently labeled carbohydrate polymer used in a variety of research applications. It has a molecular weight of 40 kilodaltons and is covalently linked to the fluorescent dye fluorescein isothiocyanate (FITC).

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Lab products found in correlation

Alexa fluor 488 conjugated secondary antibodies for facs analysis, by Thermo Fisher Scientific (1 mentions) Thp 1 cell, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Dextran 40 (9 citations) Dextrans (38 citations)

2 protocols using fitcconjugated dextran 40 kda

1

Phenotypic Analysis of THP-1 Macrophages

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THP-1 cells, purchased from American Type Culture Collection (Manassas, VA, USA) were maintained in RPMI 1640 containing 10% FBS in the presence of penicillin and streptomycin. 27OHChol and primary antibodies against CD80, CD83, CD88, CD197, and MHC molecules were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Diclofenac was purchased from ENZO Life Sciences (Farmingdale, NY, USA). FITCconjugated dextran (40 kDa) was purchased from Sigma- Aldrich (St. Louis, MO, USA). Alexa Fluor® 488-conjugated secondary antibodies for FACS analysis were purchased from Invitrogen (Eugene, Oregon, USA).
Son Y., Kim B.Y., Park Y.C, & Kim K. (2017). Diclofenac Inhibits 27-hydroxycholesterol-induced Differentiation of Monocytic Cells into Mature Dendritic Cells. Immune Network, 17(3), 179-185.
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2

Quantifying Cellular Dextran Uptake

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After treatment with reblastatins and 27OHChol, THP-1 cells were re-suspended in culture medium containing 1 mg/ml FITC-conjugated dextran (40 kDa) (Sigma Aldrich, St. Louis, MO, USA) and incubated for 30 min at 37ºC. Resultant fluorescence was analyzed using a flow cytometer.
Choi J., Kim B.Y., Son Y., Lee D., Hong Y.S., Kim M.S, & Kim K. (2020). Reblastatins Inhibit Phenotypic Changes of Monocytes/Macrophages in a Milieu Rich in 27-Hydroxycholesterol. Immune Network, 20(2), e17.
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