4–5 μm thin tissue sections from metastasis were used to conduct immunohistochemical staining. Primary antibodies against β2‐adrenergic receptor (Abcam 182136), IL6‐receptor (Abcam 128008) and COX2 (DAKO CX‐294) were used. After deparaffinisation in xylene and alcohol dilutions, the tissue sections were heated in the microwave to achieve antigen retrieval. Then, endogenous enzyme activity was blocked by peroxidase inhibition followed by application of protein block and incubation with the primary antibody. For visualisation, 3‐amino‐9‐ethylcarbazole (DAB) chromogen was added. Finally, the sections were counterstained with hematoxylin. Staining for VEGF‐A was preformed previously following the same protocol.38 The primary antibody was omitted for negative controls. Further details are presented in Table 1 .
Cx 294
Manufactured by Agilent Technologies
Sourced in Denmark
The CX‐294 is a laboratory instrument manufactured by Agilent Technologies. It is designed for sample preparation and analysis. The core function of the CX‐294 is to assist in the processing and examination of various types of samples.
Automatically generated - may contain errors
2 protocols using cx 294
1
Immunohistochemical Staining of Metastatic Tissue
2
Immunohistochemical Analysis of Endometriosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We performed immunohistochemical studies of target antigen in the serial section of biopsies derived from eutopic endometrium and from cyst walls of ovarian endometrioma using the following antibodies and concentrations: CD68 (marker of macrophages, 1:200), M0814, mouse monoclonal, Dako, Denmark; Ki-67 (cell proliferation marker, 1:100), NCL-L-Ki67-MM1, mouse monoclonal, Novocastra Laboratories Ltd, Newcastle, UK; CD31 (vascular cells marker, 1:50), M0823, mouse monoclonal, Dako, Denmark; COX2 (rate limiting enzyme for PG production, 1:100), CX-294, mouse monoclonal, Dako, Denmark; Fibronectin (cell adhesion marker, 1-400), ab2413, rabbit polyclonal, abcam, Tokyo, Japan. Non-immune immunoglobulin (Ig) G1 (1:50), a mouse monoclonal antibody from Dako, Denmark, was used as a negative control. A complete list of all antibodies, respective concentration used and positive controls is shown in Table 1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!