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Fluoromax 4c spectrofluorometer

Manufactured by Horiba
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The Fluoromax 4C spectrofluorometer is a laboratory instrument designed for the measurement and analysis of fluorescence spectra. It is capable of collecting and displaying excitation and emission spectra, as well as time-resolved fluorescence data.

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Lab products found in correlation

Cary 100 uv visible spectrophotometer, by Agilent Technologies (1 mentions) Phi 5000 versa probe 2, by Physical Electronics (1 mentions) Titan g2, by Thermo Fisher Scientific (1 mentions) Ftir spectrometer, by PerkinElmer (1 mentions) Ta q100 dsc thermal analyzer, by TA Instruments (1 mentions) Jem 2100, by JEOL (1 mentions)

3 protocols using fluoromax 4c spectrofluorometer

1

Characterization of Carbon Quantum Dots

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UV-visible electronic absorption spectra measurements were carried out using an Agilent Cary 100-UV-visible Spectrophotometer. The fluorescence emission spectra of the samples were measured using a Horiba Fluoromax 4C spectrofluorometer (HORIBA, Edison, NJ, USA). Fluorescence lifetime experiments were performed using a Horiba Jobin Yvon instrument (HORIBA, Edison, NJ, USA) in a Time-Correlated Single Photon Counting (TCSPC). A nano-LED of 390 nm was used as a source of excitation for CQDs. LUDOX AM30 (Sigma-Aldrich/ St. Louis, MO, USA) colloidal silica scattering medium was used for the instrument response function. The pulse repetition rate of TCSPC was fixed at 1 MHz. The average size of CQDs was carried out by using an FEI Titan G2 (Hillsboro, OR, USA) 300 kV high-resolution transmission electron microscope (HR-TEM). Surface functionality characterization was carried out by X-ray photoelectron spectroscopy (XPS) using PHI 5000 Versaprobe II (ULVAC-PHI, Enzo Chigasaki, Japan), FEI.
Rawat K.S., Singh V., Sharma C.P., Vyas A., Pandey P., Singh J., Gupta N.M., Sachdev M, & Goel A. (2023). Picomolar Detection of Lead Ions (Pb2+) by Functionally Modified Fluorescent Carbon Quantum Dots from Watermelon Juice and Their Imaging in Cancer Cells. Journal of Imaging, 9(1), 19.
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2

pH-Titration of DNA Switch Fluorescence

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The pH-titration curves of the pH-sensitive DNA switch (100 nM; STAR Methods) were conducted in a universal citrate/phosphate/borate buffer whose pH was adjusted to the desired value. The solutions of the universal buffer at different pH were prepared according to a previously reported method.70 (link) Equilibrium fluorescence measurements were obtained using a Fluoromax-4C spectrofluorometer (Horiba) with excitation at 488 nm and acquisition between 540 and 650 nm at 25°C. The normalized fluorescence data F (at 572 nm) was fitted with the following equation: F= F0+ (Fm-F0) × 10−k/(10−k+10−x).48 (link) In this equation, F0 is the minimum fluorescence signal (triplex state); Fm is the maximum fluorescence signal (duplex state); k is the pH of the inflection point, and x is the pH of the solution.
Peng H., Lelievre A., Landenfeld K., Müller S, & Chen I.A. (2021). Vesicle encapsulation stabilizes intermolecular association and structure formation of functional RNA and DNA. Current biology : CB, 32(1), 86-96.e6.
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3

Multimodal Characterization of Nanomaterials

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The absorption spectra
were taken using
a Varian model Cary wins Bio 100 spectrometer in the range of 200–800
nm. The fluorescence emission spectra were recorded using the Fluoro
Max-4C spectrofluorometer (Horiba Instruments, USA). The slits for
excitation and emission were set at 5 nm. Lifetime analysis was measured
using time-correlated single photon counting with a pulse width of
1.3 ns. High-resolution transmission electron microscopy (HRTEM) images
were recorded on a JEOL JEM 2100 instrument with an acceleration voltage
of 200 kV. An Omicron ESCA Probe spectrometer with unmonochromatized
Mg Kα X-rays was used for X-ray photoelectron spectroscopy (XPS)
analysis. Fourier-transform infrared (FTIR) spectra were recorded
using a PerkinElmer FTIR spectrometer. Thermal transition measurements
were done using a TA Q100-DSC thermal analyzer (TA Instruments, New
Castle, Delaware 19720 USA).
Mathew M.S., Vinod K., Jayaram P.S., Jayasree R.S, & Joseph K. (2019). Improved Bioavailability of Curcumin in Gliadin-Protected Gold Quantum Cluster for Targeted Delivery. ACS Omega, 4(10), 14169-14178.
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