Model qp 2010 series

GC-MS analysis was performed on Model QP 2010 series, Shimadzu, Tokyo, Japan, equipped with AOC-20i auto sampler and RTX-1 fused silica capillary column (30 m length, 0.25 mm id, and 0.25 µm thickness). Helium gas (purity 99.99%) was used as a carrier gas at a flow rate of 1.5 ml/min. The injector temperature was fixed at 280°C and samples were injected through split injection mode. The column oven program was set at 50°C for 2 min, then increased to 28°C with the rate of 10°C/min. Ion source temperature was applied to 230°C and interface temperature was set to 250°C. The mass range from 36 to 800 m/z was scanned at a rate of 3.0 scans/s. The total run time of GC-MS system was 36 min (Patel and Ghane, 2021 (link)). Compounds were identified by comparison with authentic spectra obtained from GC-MS library (NIST 11).

The dried algal biomass (100 mg) of Pectinodesmus sp. (PHM3) was extracted using ethanol and water in 50:50 ratio. The impure organic part obtained from extraction was sieved using 0.22 μm membrane filter and concentrated to dryness using rotary evaporator machine at low pressure. The fraction obtained after evaporation was examined through GC-MS [48 ]. The equipment used was a Model QP 2010 series from Shimadzu, Tokyo, Japan. The heat was programmed to rise from 50ºC to 300ºC with 2ºC rise per minute. Sample components were ionized in electron impact mode (EI, 70 eV). The temperature of the detector and injector was fixed at 310ºC and 300ºC, respectively, carrier gas Helium was used with a purity of 99.995%. The flow rate was set at 1ml/min with the scanning rate at 3.0 scan/s and mass range of 40 to 1000 m/z. One microliter of source from PHM3 was injected into the GC-MS machine with the help of a Hamilton syringe using split injection (1:40) for total ionic chromatographic analysis. The GC-MS machine was run for 15 min and the analog data was converted to digital data through GC solution software.