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Ssdonors is a specialized lab equipment product from Integrated DNA Technologies. It is designed for the collection and storage of single-stranded DNA samples.

Automatically generated - may contain errors

Lab products found in correlation

Cas9 ribonucleoprotein complex, by Integrated DNA Technologies (2 mentions) Guide rna, by Integrated DNA Technologies (2 mentions) Tracrrna, by Integrated DNA Technologies (2 mentions)

2 protocols using ssdonors

1

CRISPR-mediated Genome Editing in C. elegans

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
CRISPR lines were generated via Cas9 editing using short single-stranded oligo donors (ssdonors) as previously reported (Ghanta and Mello, 2020 (link)). In brief, two ssdonors were used to introduce synonymous mutations and deletion at target site of 21UR-4864 of w03h9.2. 20 μl injection mix contained pre-assembled Cas9 ribonucleoprotein complex (5 μg Cas9, 2 μg guide RNA, 1 μg tracrRNA) and 1.1 μg ssdonors (Integrated DNA Technologies). The vector pRF4 was used as a co-injection marker (Mello et al., 1991 (link)). Sequences of guide RNA and ssdonors used in this study are listed in Table S5.
Pastore B., Hertz H.L., Price I.F, & Tang W. (2021). pre-piRNA trimming and 2′-O-methylation protect piRNAs from 3′ tailing and degradation in C. elegans. Cell reports, 36(9), 109640.
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2

CRISPR-mediated Genome Editing in C. elegans

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
CRISPR lines were generated via Cas9 editing using short single-stranded oligo donors (ssdonors) as previously reported (Ghanta and Mello, 2020 (link)). In brief, two ssdonors were used to introduce synonymous mutations and deletion at target site of 21UR-4864 of w03h9.2. 20 μl injection mix contained pre-assembled Cas9 ribonucleoprotein complex (5 μg Cas9, 2 μg guide RNA, 1 μg tracrRNA) and 1.1 μg ssdonors (Integrated DNA Technologies). The vector pRF4 was used as a co-injection marker (Mello et al., 1991 (link)). Sequences of guide RNA and ssdonors used in this study are listed in Table S5.
Pastore B., Hertz H.L., Price I.F, & Tang W. (2021). pre-piRNA trimming and 2′-O-methylation protect piRNAs from 3′ tailing and degradation in C. elegans. Cell reports, 36(9), 109640.
+ Open protocol
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