U-937 (human histiocytic lymphoma monocyte; ATCC CRL-1593.2) cells (Sundström and Nilsson 1976 (link)) were cultured in RPMI-1640 medium (Corning® glutagro™ 10-104-CV) with 10% fetal bovine serum (Corning, 35-010-CV) and 1% penicillin/streptomycin (10,000 U mL−1 penicillin and 10 mg mL−1 streptomycin) at 37 °C in a humidified, 5% CO2 atmosphere.
Glutagro 10 104 cv
Manufactured by Corning
Glutagro™ 10-104-CV is a specialized laboratory equipment designed for cell culture applications. It is a cell culture vessel with a growth surface area of 104 cm2. The product is constructed with a polystyrene material and features a vented cap for gas exchange.
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Lab products found in correlation
3 protocols using glutagro 10 104 cv
1
Culturing U-937 Monocyte-like Cells
2
Culturing U-937 Cells for Microscopy
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U-937 (human histiocytic lymphoma monocyte; ATCC CRL-1593.2) cells65 (link) were cultured in RPMI-1640 medium (Corning® glutagro™ 10-104-CV) with 10% fetal bovine serum (Corning, 35-010-CV) and 1% penicillin/streptomycin (10000 U/mL penicillin and 10 mg/mL streptomycin) at 37 °C in a humidified, 5% CO2 atmosphere. For microscope observations cells were washed and suspended at approximately 5 × 105 cells/mL in fresh medium containing 2 µM YO-PRO-1 (Molecular Probes) for experiments.
3
Culturing U-937 Monocyte Cell Line
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U-937 (human histiocytic lymphoma monocyte; ATCC CRL-1593.2) cells [34 (link)] were cultured in RPMI-1640 medium (Corning® glutagro™ 10–104-CV) with 10% fetal bovine serum (Corning, 35–010-CV) and 1% penicillin/streptomycin at 37 °C in a humidified, 5% CO2 atmosphere.
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