Hemoglobin standard

Manufactured by Merck Group

Sourced in United States

The Hemoglobin standard is a laboratory reference material used to calibrate and validate the performance of instruments that measure hemoglobin levels in blood samples. It provides a known concentration of hemoglobin to ensure the accurate and reliable quantification of hemoglobin in clinical and research applications.

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Lab products found in correlation

Drabkin s solution, by Merck Group (2 mentions) Cd8 apc, by BioLegend (1 mentions) Cd4 fitc, by BioLegend (1 mentions) Cd86 pe, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Sartorius (1 mentions) Mhcii fitc, by BioLegend (1 mentions) Cd3 percp cyanine5, by BioLegend (1 mentions) Heat inactivated fbs, by Sartorius (1 mentions) Penicillin, by Beyotime (1 mentions) Streptomycin, by Beyotime (1 mentions) Goat serum, by Beyotime (1 mentions) Mtt cell proliferation and cytotoxicity assay kit, by Beyotime (1 mentions) Drabkin s reagent kit, by Merck Group (1 mentions) 96 well round bottom plate, by BD (1 mentions) Elx808, by Agilent Technologies (1 mentions) Gfr matrigel matrix, by BD (1 mentions) Red blood cell lysis solution, by Merck Group (1 mentions) Growth factor reduced matrigel, by Corning (1 mentions) Rbc lysis buffer, by Merck Group (1 mentions) Drabkin s reagent, by Merck Group (1 mentions)

6 protocols using hemoglobin standard

Alginate-Dopamine Hydrogel for Angiogenesis

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Alginate sodium (160 mPa·s viscosity) with low G/M ratio was purchased from Qingdao Crystal Salt Bioscience and Technology Corporation (China). The G/M ratio of the alginate sodium was 1:3, as determined by circular dichroism (CD) spectrometer.19 (link) Endostar was bought from Shandong Xiansheng Maidejin Biological Pharmaceutical Co., Ltd (China). Dopamine hydrochloride was purchased from Yuancheng Technology Development Co., Ltd. (China). Mouse VEGF, matrix metalloproteinase-9 (MMP-9), IL-8 and IL-17 ELISA kits were obtained from Shanghai Enzyme-Linked Biotechnology Co., Ltd. (China). Mouse IFN‑γ, TNF-α, IL-4, IL-6, IL-10 and IL-12 ELISA kits, and CD11c-PerCP-Cyanine5.5, MHCI-APC and CD86-PE were the products of eBioscience (USA). Mouse monoclonal antibodies MHCII-FITC, CD3-PerCP/Cyanine5.5, CD8-APC and CD4-FITC were purchased from BioLegend (USA). MTT Cell Proliferation and Cytotoxicity Assay Kit, penicillin, streptomycin and goat serum were purchased from Beyotime (China). In situ Cell Death Detection Kit (POD), Drabkin’s reagent, and hemoglobin standard were obtained from Sigma-Aldrich, Inc. (USA). RPMI-1640 medium and heat-inactivated FBS were purchased from Biological Industries (Israel).

Yang Y., Wang N., Tian X., Wang X., Yang J., Leng X, & Zhang H. (2022). Synergy of Polydopamine Nanovaccine and Endostar Alginate Hydrogel for Improving Antitumor Immune Responses Against Colon Tumor. International Journal of Nanomedicine, 17, 4791-4805.

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Tumor Angiogenesis Assessment in Mice

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To evaluate tumor-induced angiogenesis, PC-3 cells (3 × 10⁶/100 μl serum-free medium) mixed in Matrigel (500 μl) with heparin (50 U/ml) and WEF were subcutaneously injected into the abdomen of athymic nude mice. At day 14 after implantation, Matrigel plugs were carefully removed and hemoglobin content in gel plugs was determined using a Drabkin's reagent kit (Sigma) as described previously30 (link). The concentration of hemoglobin was calculated based on the set of hemoglobin standard (Sigma).

Kim A., Im M., Yim N.H, & Ma J.Y. (2014). Reduction of metastatic and angiogenic potency of malignant cancer by Eupatorium fortunei via suppression of MMP-9 activity and VEGF production. Scientific Reports, 4, 6994.

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Quantification of Serum Iron and Hemoglobin

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Total serum iron was measured using the IRON FZ kit (Chema Diagnostics) as recommended by the suppliers. Serum MPO concentrations were measured as described by the suppliers (R&D systems). Blood Hemoglobin levels were quantified colorimetrically. Briefly, 2 µl blood was diluted in 200 µl distilled water in a 96 well round bottom plate (Falcon) and incubated for 30 min. at 37°C. After centrifugation (600 g, 10 min.) the supernatant was collected and the OD540nm measured in an Ultra Microplate reader (ELx808, Bio-Tek instruments.inc). The hemoglobin concentration was calculated using a hemoglobin standard (Sigma).

Stijlemans B., Leng L., Brys L., Sparkes A., Vansintjan L., Caljon G., Raes G., Van Den Abbeele J., Van Ginderachter J.A., Beschin A., Bucala R, & De Baetselier P. (2014). MIF Contributes to Trypanosoma brucei Associated Immunopathogenicity Development. PLoS Pathogens, 10(9), e1004414.

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Angiogenesis Assay Using Matrigel Plug

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0.5 mL Matrigel Matrix GFR (growth factor reduced)-PRF (phenol red free) (BD Biosciences) solution was mixed with 1 μg FGF2 and subcutaneously implanted into the right flank of female C57BL/6J mice (8 weeks, n = 3). RBM-007 was administered intraperitoneally every day at doses of 1, 3, and 10 mg/kg, and Matrigel plugs were removed and photographed on day 7 post-implantation. Grading of angiogenesis was determined by the hemoglobin concentration in the Matrigel plug, using the cyanmethemoglobin method according to the manufacturer’s instructions. In brief, the Matrigel plug was macerated with an equal volume of red blood cell (RBC) lysis solution (Sigma-Aldrich) and incubated overnight on ice. The preparation samples were added to Drabkin’s solution (Sigma-Aldrich) containing 0.3% Brij-35 and incubated for 30 min at room temperature. The optical density at 540 nm (OD₅₄₀) values of the samples were measured in a microplate reader, and the hemoglobin concentrations were calculated in accordance with hemoglobin standards (Sigma-Aldrich).

Matsuda Y., Nonaka Y., Futakawa S., Imai H., Akita K., Nishihata T., Fujiwara M., Ali Y., Bhisitkul R.B, & Nakamura Y. (2019). Anti-Angiogenic and Anti-Scarring Dual Action of an Anti-Fibroblast Growth Factor 2 Aptamer in Animal Models of Retinal Disease. Molecular Therapy. Nucleic Acids, 17, 819-828.

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Quantifying In Vivo Angiogenesis via Matrigel Plug Assay

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The Matrigel plug assay was performed to evaluate the angiogenic response in vivo. Matrigel Growth Factor Reduced (0.45 mL, Corning) mixed with heparin (10 U/mL) and PBS or BafA-PD (500 ng/mL) were subcutaneously injected into the flanks of 8-week-old C57BL/6J mice. On day 10 post injection, mice were sacrificed, Matrigel plugs were removed, and blood vessel formation was evaluated by quantification of hemoglobin. The hemoglobin assay was performed as follows: Matrigel plugs were soaked in an equal volume of RBC lysis buffer (Sigma-Aldrich) and incubated overnight on ice. Drabkin’s solution (Sigma-Aldrich) containing 0.3% Brij-35 was added to the samples and incubated for 30 min at room temperature. The hemoglobin concentration was determined by comparing the spectral absorption at 540 nm of the sample with that of hemoglobin standards (Sigma-Aldrich).

Tsukamoto K., Shinzawa N., Kawai A., Suzuki M., Kidoya H., Takakura N., Yamaguchi H., Kameyama T., Inagaki H., Kurahashi H., Horiguchi Y, & Doi Y. (2020). The Bartonella autotransporter BafA activates the host VEGF pathway to drive angiogenesis. Nature Communications, 11, 3571.

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Matrigel-Plug Angiogenesis Assay in Mice

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Matrigel-plug angiogenesis was performed as described [27 (link)]. Mice (six animals for each experimental group) were injected with matrigel (10 mg/ml), VEGF (100 ng) plus a control retrovirus shRNA or PDCL3 shRNA. Retroviruses were prepared as described [24 (link)]. The animals were sedated with Avertin (0.3 ml per 20-g mouse), and a 25-gauge needle was used to inject matrigel mixture (200 μl) subdermally into mice. After 8 days, animals were killed, matrigel plugs were removed, and plugs were homogenized in 1 ml of deionized water on ice and cleared by centrifugation at 10,000 rpm for 5 min at 4 °C. The supernatant was collected and used to measure hemoglobin content with Drabkin’s reagent along with hemoglobin standards as suggested by the manufacturer (Sigma-Aldrich, St. Louis, MO), and the absorbance was read at 540 nm [28 (link)].

Srinivasan S., Chitalia V., Meyer R.D., Hartsough E., Mehta M., Harrold I., Anderson N., Feng H., Smith L.E., Jiang Y., Costello C.E, & Rahimi N. (2015). Hypoxia-induced expression of phosducin-like 3 regulates expression of VEGFR-2 and promotes angiogenesis. Angiogenesis, 18(4), 449-462.

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